Which method is better for copy number variation detection, NGS or microarray?# Biology - 生物学
c*e
1 楼
我几次读到报道，说用next generation sequencing 进行copy number variant的检测
技术还不成熟，因此，high throughput 的copy number variation检测还是用
microarray. 然而，报道的只是个结论，并没有说原因。
版上都是生物技术前沿的斗士们，一定有人会有一些经验和前瞻性的建议。所以，我特
来请教:
1) how microassay is better than next generation sequencing on copy number
detection.
2) If we have a sequencing assay for a targeted gene panel by next
generation sequencing, and want to further detect the copy number variation
on the same genes, what improvement is needed before using the NGS data for
copy number variant analysis?
3) Any recommendation on software using NGS data for copy number variant
analysis？
Thank you very much!
技术还不成熟，因此，high throughput 的copy number variation检测还是用
microarray. 然而，报道的只是个结论，并没有说原因。
版上都是生物技术前沿的斗士们，一定有人会有一些经验和前瞻性的建议。所以，我特
来请教:
1) how microassay is better than next generation sequencing on copy number
detection.
2) If we have a sequencing assay for a targeted gene panel by next
generation sequencing, and want to further detect the copy number variation
on the same genes, what improvement is needed before using the NGS data for
copy number variant analysis?
3) Any recommendation on software using NGS data for copy number variant
analysis？
Thank you very much!
