Measure glucose level in mouse serum# Biology - 生物学
t*i
1 楼
开头那个真是整齐啊。虽然这个trick没啥用，不过挺好玩的。
另外这个作者还教了她家的狗狗很多有意思的trick，有兴趣大家可以去看一下
另外这个作者还教了她家的狗狗很多有意思的trick，有兴趣大家可以去看一下
r*a
2 楼
Hi,
I treated groups of transgenic mice with a few chemicals for inhibiting
cancer but noticed one chemical may reduce the activity of insulin. A
direct consequence should be higher blood glucose level/increased insulin.
But the experiment has been completed and there is not much chance to
convince my boss to repeat an experiment for measure blood glucose level. I
am not in the metabolism field but found that most works in the
publications measure the glucose after overnight fasting or at least using
fresh blood drops. Can I use the frozen serum (collected for measure
cytokines) to measure blood glucose levels?
Thanks!
I treated groups of transgenic mice with a few chemicals for inhibiting
cancer but noticed one chemical may reduce the activity of insulin. A
direct consequence should be higher blood glucose level/increased insulin.
But the experiment has been completed and there is not much chance to
convince my boss to repeat an experiment for measure blood glucose level. I
am not in the metabolism field but found that most works in the
publications measure the glucose after overnight fasting or at least using
fresh blood drops. Can I use the frozen serum (collected for measure
cytokines) to measure blood glucose levels?
Thanks!
m*h
3 楼
Q~~~
r*g
4 楼
should be ok in theory. But I have never tried frozen serum. Just buy a
glucose meter from pharmacy. It works fine in our hands for mice serum.
glucose meter from pharmacy. It works fine in our hands for mice serum.
D*a
5 楼
测糖尿病人的血糖仪，尾巴拿针头扎一下，挤血，捏到血糖仪纸条上。
一只老鼠可以给药前后测，还可以隔几分钟、几小时、天天测，statistic power大大
好于你去冰箱里面捞血清。
找个你们楼做糖尿病的实验室去聊聊。
血清也不是不行，但是糖尿病血糖仪有校准，你拿它测血清和拿它测全血不能直接比。
一只老鼠可以给药前后测，还可以隔几分钟、几小时、天天测，statistic power大大
好于你去冰箱里面捞血清。
找个你们楼做糖尿病的实验室去聊聊。
血清也不是不行，但是糖尿病血糖仪有校准，你拿它测血清和拿它测全血不能直接比。
D*a
6 楼
不fasting的问题是老鼠吃饭都不定点，浮动明显比fasting的大，不过也还可以，你药
明显的话肯定没问题，如果你要fasting，记得换新笼子，因为老鼠吃粑粑
明显的话肯定没问题，如果你要fasting，记得换新笼子，因为老鼠吃粑粑
l*y
7 楼
找个做diabetes的问问，测这个都是routine的
I
【在 r***a 的大作中提到】
: Hi,
: I treated groups of transgenic mice with a few chemicals for inhibiting
: cancer but noticed one chemical may reduce the activity of insulin. A
: direct consequence should be higher blood glucose level/increased insulin.
: But the experiment has been completed and there is not much chance to
: convince my boss to repeat an experiment for measure blood glucose level. I
: am not in the metabolism field but found that most works in the
: publications measure the glucose after overnight fasting or at least using
: fresh blood drops. Can I use the frozen serum (collected for measure
: cytokines) to measure blood glucose levels?
I
【在 r***a 的大作中提到】
: Hi,
: I treated groups of transgenic mice with a few chemicals for inhibiting
: cancer but noticed one chemical may reduce the activity of insulin. A
: direct consequence should be higher blood glucose level/increased insulin.
: But the experiment has been completed and there is not much chance to
: convince my boss to repeat an experiment for measure blood glucose level. I
: am not in the metabolism field but found that most works in the
: publications measure the glucose after overnight fasting or at least using
: fresh blood drops. Can I use the frozen serum (collected for measure
: cytokines) to measure blood glucose levels?
h*o
8 楼
serum glucose测试没问题， frozen sample也可以用
但是如果你当时sac 老鼠的时候没有fast， 那么需要很大的N per group 才能检测到
overall的区别
老鼠隔三差五就会去啃一口mouse chow， 所以blood glucose 变化会比较大 除非你的
药像streptozotocin这种强效。
但是如果你当时sac 老鼠的时候没有fast， 那么需要很大的N per group 才能检测到
overall的区别
老鼠隔三差五就会去啃一口mouse chow， 所以blood glucose 变化会比较大 除非你的
药像streptozotocin这种强效。
W*7
9 楼
Go ahead give a try. Should be fine. If you do have any problem, we have a
machine for measuring glucose level in serum.
machine for measuring glucose level in serum.
C*u
10 楼
我试过用serum测过glucose，大概比用新鲜的血测高一倍左右。
应该是因为血里面大概一半的体积是血细胞。
I
【在 r***a 的大作中提到】
: Hi,
: I treated groups of transgenic mice with a few chemicals for inhibiting
: cancer but noticed one chemical may reduce the activity of insulin. A
: direct consequence should be higher blood glucose level/increased insulin.
: But the experiment has been completed and there is not much chance to
: convince my boss to repeat an experiment for measure blood glucose level. I
: am not in the metabolism field but found that most works in the
: publications measure the glucose after overnight fasting or at least using
: fresh blood drops. Can I use the frozen serum (collected for measure
: cytokines) to measure blood glucose levels?
应该是因为血里面大概一半的体积是血细胞。
I
【在 r***a 的大作中提到】
: Hi,
: I treated groups of transgenic mice with a few chemicals for inhibiting
: cancer but noticed one chemical may reduce the activity of insulin. A
: direct consequence should be higher blood glucose level/increased insulin.
: But the experiment has been completed and there is not much chance to
: convince my boss to repeat an experiment for measure blood glucose level. I
: am not in the metabolism field but found that most works in the
: publications measure the glucose after overnight fasting or at least using
: fresh blood drops. Can I use the frozen serum (collected for measure
: cytokines) to measure blood glucose levels?
z*t
11 楼
我用frozen serum做过metablomics没有fasting的情况下没有特别大的variation，抽
血的时间也不定时
不过我是做的leukemia mice， 跟control进行比较，如果要测很准的血糖还是要小心
I
【在 r***a 的大作中提到】
: Hi,
: I treated groups of transgenic mice with a few chemicals for inhibiting
: cancer but noticed one chemical may reduce the activity of insulin. A
: direct consequence should be higher blood glucose level/increased insulin.
: But the experiment has been completed and there is not much chance to
: convince my boss to repeat an experiment for measure blood glucose level. I
: am not in the metabolism field but found that most works in the
: publications measure the glucose after overnight fasting or at least using
: fresh blood drops. Can I use the frozen serum (collected for measure
: cytokines) to measure blood glucose levels?
血的时间也不定时
不过我是做的leukemia mice， 跟control进行比较，如果要测很准的血糖还是要小心
I
【在 r***a 的大作中提到】
: Hi,
: I treated groups of transgenic mice with a few chemicals for inhibiting
: cancer but noticed one chemical may reduce the activity of insulin. A
: direct consequence should be higher blood glucose level/increased insulin.
: But the experiment has been completed and there is not much chance to
: convince my boss to repeat an experiment for measure blood glucose level. I
: am not in the metabolism field but found that most works in the
: publications measure the glucose after overnight fasting or at least using
: fresh blood drops. Can I use the frozen serum (collected for measure
: cytokines) to measure blood glucose levels?
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