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关于AF488标记的antagonist在flow cytometry上面的问题,谢谢!
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关于AF488标记的antagonist在flow cytometry上面的问题,谢谢!# Biology - 生物学
S*h
1
It is from Amazon telephone interview. The traditional question:
intersection of two lists. He wanted me to think about alternative of
hashtable. So build a search tree. He then asked what happened if the
list
is so large that it has to be stored across a cluster. How would you
store
this search tree? Any good idea?
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m*d
2
【 以下文字转载自 WaterWorld 讨论区 】
发信人: happydad (R2R--》 C2C---》Mt.whitney), 信区: WaterWorld
标 题: 难怪林彪被叛国,原来他老婆是韩国人!(转)
发信站: BBS 未名空间站 (Thu Sep 8 21:27:31 2011, 美东)
大家看看像不像,呵呵
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t*n
3
一个荧光(Alexa Fluor 488)标记的antagonist可以抑制细胞表面的分子信号通路,
IC50在100~200nM。但是用这个AF488标记的antagonist染色target高表达的细胞系,
在flow cytometry上面看不见那个波峰向右边移动,请问大侠可能的原因是什么?谢谢
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q*x
4
maybe he means bst not the right answer.

【在 S****h 的大作中提到】
: It is from Amazon telephone interview. The traditional question:
: intersection of two lists. He wanted me to think about alternative of
: hashtable. So build a search tree. He then asked what happened if the
: list
: is so large that it has to be stored across a cluster. How would you
: store
: this search tree? Any good idea?

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c*8
5
你的抗体不work 吧!
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S*h
6
No. We have already discussed about hashtable. On a cluster environment, I
told him that we might want to do a two-level hash, first map to one node.
He seemed not against it. Then he said, let us switch the gear and looks at
alternative to hash. For BST, I said, we can do make something like
multilevel hash, first map to one node with a big branch. Then within the
node use BST. He seems to have something more in mind.
He is from AWS unit. That seems a very relevant question for them.
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s*c
7
如果你确定你的小分子结合你想看的target,那么最大的可能是dissociation太快。小
分子和抗体的结合动力学有时候会差很多,描述小分子作用方式的不能仅仅用affinity
或IC50,小分子哪怕affinity在single digit nM range,可能dissociation都是秒级
的,这样在FACS里当然看不到。
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t*n
8
谢谢回复!AF488标记应该work,高浓度的时候能看见非特异的结合。
请问seraphzc,有什么办法可以解决这个off rate太快的问题?我试了Formaldehyde
fix,对小分子好像不起作用,还有啥其他得可能方法吗?
非常感谢!

affinity

【在 s******c 的大作中提到】
: 如果你确定你的小分子结合你想看的target,那么最大的可能是dissociation太快。小
: 分子和抗体的结合动力学有时候会差很多,描述小分子作用方式的不能仅仅用affinity
: 或IC50,小分子哪怕affinity在single digit nM range,可能dissociation都是秒级
: 的,这样在FACS里当然看不到。

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