Re: how to avoid contamination when doing PCR?# Biology - 生物学
b*z
1 楼
there are so many things that can go wrong in a pcr expt. your purpose would
be defined to carry out an expt without appreciable contamination rather
to figure out what has gone wrong.
typically if you want to find what's wrong, you would change one variables
(any buff, pcr station, even a pipette can be considered a single varibable)
at one time, repeat pcr expt and figure out what has gone wrong.
I would not do that though. you only have to figure out whether your own pcr
station has contamina
be defined to carry out an expt without appreciable contamination rather
to figure out what has gone wrong.
typically if you want to find what's wrong, you would change one variables
(any buff, pcr station, even a pipette can be considered a single varibable)
at one time, repeat pcr expt and figure out what has gone wrong.
I would not do that though. you only have to figure out whether your own pcr
station has contamina