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My opinion

My opinion# Biology - 生物学

l*k2001-05-15 07:05

1 楼

I think DNA microarray will SOON be out of date (my personal
opinion, don't feel offended) -- not the reason mentioned by
musician, but because I think
soon the test will be saturated and a more systematic way
for data analysis will
emerge, by that time, most microarray facility will find
nothing to do. Basically, microarray has to be combined with
other techniques...
As to success research using DNA chip, there are several
very good papers out there. One in cell, about a yeast
expression compen

a*a2001-05-15 07:05

2 楼

技术发展很难预计的。
不知道你这里microarray和DNA chip有什么区别，指PCR的print
和oligo Array之间的差别么？如果是这样的话
microarray较affy chip存在的理由，是PCR product的print并不需要
知道完全的序列，而chip制造的基础在于你需要知道sequence,构建
oligo库，制造芯片。
从数据的角度而言，两者的数据分析都存在着极大的variance, 所以实际
上，现在能利用的数据只是得到的数据的冰山一角而已。看过pat brown的
paper么？不做repeat, 做上上千种条件。对于每一张slide, 数据实际上
没有意义的，但是当把上千张slide cluster到一块的时候，这些数据就变
得有意义了。
从零一个角度而言，从现有的分析中，我们只能关注那些极端express或者
repress的基因，就如你列的那些文章，作为一般实验技术的前导与辅助，
去研究某个具体的基因，具体的条件而已，但这只是microarray/chip运用
的一个方面而已
如何能提高技术的精确度，如何能运用大量不是特别极端的数据，这是

【在 l*****k 的大作中提到】

: I think DNA microarray will SOON be out of date (my personal
: opinion, don't feel offended) -- not the reason mentioned by
: musician, but because I think
: soon the test will be saturated and a more systematic way
: for data analysis will
: emerge, by that time, most microarray facility will find
: nothing to do. Basically, microarray has to be combined with
: other techniques...
: As to success research using DNA chip, there are several
: very good papers out there. One in cell, about a yeast