Re: about primer# Biology - 生物学
d*o
1 楼
From which company did you order your primer?
we order from Invitrogen, and they use different salt concentration
to calculate Tm. It's not about right or wrong, coz Tm changes with
oligonucleotide and salt concentrations, so u'd better calculate it by
yourself. Some softwares like Vector NTI can do it, I think Primer3
works too.
we use oligo conc. at 250 pM and salt conc. at 250 pM to calculate
the Tms.
we order from Invitrogen, and they use different salt concentration
to calculate Tm. It's not about right or wrong, coz Tm changes with
oligonucleotide and salt concentrations, so u'd better calculate it by
yourself. Some softwares like Vector NTI can do it, I think Primer3
works too.
we use oligo conc. at 250 pM and salt conc. at 250 pM to calculate
the Tms.