Re: 关于characterize a protein的问题求助# Biology - 生物学
l*k
1 楼
my understanding of your question is:
the protein you are interested in, say LHRH, has N terminal sequence
identical as protein A. Now you want to use RT-PCR to get the cDNA for
this protein.
I am not sure if protein A is also in your organism, if not, RT-PCR might
work, if yes, RT-PCR won't work as you will get cDNA for protein A...
I personally think you should try two methods, maybe 3 to be sure:
1. RT-PCR.
2. cDNA library
3. Genomic library.
method one is easier to try, but hard to be sure,
the protein you are interested in, say LHRH, has N terminal sequence
identical as protein A. Now you want to use RT-PCR to get the cDNA for
this protein.
I am not sure if protein A is also in your organism, if not, RT-PCR might
work, if yes, RT-PCR won't work as you will get cDNA for protein A...
I personally think you should try two methods, maybe 3 to be sure:
1. RT-PCR.
2. cDNA library
3. Genomic library.
method one is easier to try, but hard to be sure,