Re: 厚着脸皮问一句：1 OD到底对应多大浓度的单链DNA溶液？# Biology - 生物学
M*e
1 楼
make sure that you are aware of the buffer effect:
It is estimated that DNA has 15% lower absorbance in TE
buffer, 23% lower in TE+saline compared to water.
1 O.D.260 are 38, 45 and 50 ug/ml for DNA in ddH2O,
TE and TES, respectively. I assume the same for RNA,
and 1 O.D.260 are 40 ug/ml for RNA in TE (from Ambion).
btw, A260/A280 is different for pure RNA
DEPC'd H2O (pH5-6) 1.60
Nuclease free H2O (pH6-7) 1.85
TE (pH8.0) 2.14
It is estimated that DNA has 15% lower absorbance in TE
buffer, 23% lower in TE+saline compared to water.
1 O.D.260 are 38, 45 and 50 ug/ml for DNA in ddH2O,
TE and TES, respectively. I assume the same for RNA,
and 1 O.D.260 are 40 ug/ml for RNA in TE (from Ambion).
btw, A260/A280 is different for pure RNA
DEPC'd H2O (pH5-6) 1.60
Nuclease free H2O (pH6-7) 1.85
TE (pH8.0) 2.14