Re: How long does it take for expression# Biology - 生物学
m*o
1 楼
The time for assay your transient expression depends on your host cell, transcrioption
effeciency,posttranslation mechanism etc. In one words, there are many may have
effect on your expression assay. So people used to do a progressive assay to
determine the best time point for your assay.
According to different transfection protocol, there is difference of incubation
time before conducting the assay.
The mechanism of transfection between Lipo and Calcium is diferent. For the
different cases, the
effeciency,posttranslation mechanism etc. In one words, there are many may have
effect on your expression assay. So people used to do a progressive assay to
determine the best time point for your assay.
According to different transfection protocol, there is difference of incubation
time before conducting the assay.
The mechanism of transfection between Lipo and Calcium is diferent. For the
different cases, the