Re: Question about immunofluorescence# Biology - 生物学
r*o
1 楼
It is OK! Actually that's the whole basis for FACS analysis.
The point of fixation is not to denature the protein. It just
crosslinks the proteins and make the organelle structures stable in
a cell.
The antibodies used in IF are usually raised against antigen
in its native structure.
The point of fixation is not to denature the protein. It just
crosslinks the proteins and make the organelle structures stable in
a cell.
The antibodies used in IF are usually raised against antigen
in its native structure.