Re: ask a question about mass spectrometry# Biology - 生物学
h*n
1 楼
This approach is good in theory but whether it will work or not
largely depends on your luck and how well all these experiments
are performed.
I guess you have the sequence of this protein and you have some
idea of which try residues may be phosphorylated.
First thing you want to do is to pufify your protein and then
probe your protein with antibody specific to pY on western blot.
If you see it lights up on the western blot, most likely you
have some pY residues in your protein.
Then you proceed
largely depends on your luck and how well all these experiments
are performed.
I guess you have the sequence of this protein and you have some
idea of which try residues may be phosphorylated.
First thing you want to do is to pufify your protein and then
probe your protein with antibody specific to pY on western blot.
If you see it lights up on the western blot, most likely you
have some pY residues in your protein.
Then you proceed
