哦,我没比较过,因为我一般不需要把结合上去的蛋白再洗下来。 我就是看说明书上这么说的: 2.2 Release of Immobilized Biotinylated Molecules The biotin-streptavidin bond is broken by harsh conditions. 5 mins incubation at 65°C or 2 mins at 90°C in 10 mM EDTA pH 8.2 with 95% formamide will typically dissociate >96% of immobilized biotinylated DNA. Alternatively, boil the sample for 5 mins in 0.1% SDS for protein dissociation. Please note that proteins will be denatured by such treatment and Dynabeads Streptavidin can not be re-used. It has al