a*s
2 楼
We know now that the binding between histone and DNA is specific and non-
sequence specific.Do you guys know how to prove that the histone-DNA binding
is specific, not non-specific? especially at the initial stage of this
study. I couldn't find any paper. Thanks a lot.
sequence specific.Do you guys know how to prove that the histone-DNA binding
is specific, not non-specific? especially at the initial stage of this
study. I couldn't find any paper. Thanks a lot.
I*y
4 楼
make yourself clear first
binding
【在 a******s 的大作中提到】
: We know now that the binding between histone and DNA is specific and non-
: sequence specific.Do you guys know how to prove that the histone-DNA binding
: is specific, not non-specific? especially at the initial stage of this
: study. I couldn't find any paper. Thanks a lot.
binding
【在 a******s 的大作中提到】
: We know now that the binding between histone and DNA is specific and non-
: sequence specific.Do you guys know how to prove that the histone-DNA binding
: is specific, not non-specific? especially at the initial stage of this
: study. I couldn't find any paper. Thanks a lot.
E*A
5 楼
我用高压锅,上气后调小火半小时
A*y
6 楼
http://www.ncbi.nlm.nih.gov/pubmed/15100411
Not so sure what are you asking, but here is a paper should answer some of
your questions.
Not so sure what are you asking, but here is a paper should answer some of
your questions.
a*s
8 楼
Thanks. I am trying to clarify my question.For most of transcription factors
, there is a specific binding site (consensus sequences) on DNA for them to
bind. Without them, no binding. We can use ChIP or EMSA to test it. However,
for histone, there is no specific binding site on DNA. But histone binds to
any DNA. So we call that the histone-DNA binding is specific, but non-
sequence specific.But for some proteins, they may have some kind of
association, but not direct binding with DNA. So my question is, how to
demonstrate that the binding between histone and DNA is a direct binding?
How the binding ability of histone to DNA was found and proved? Or in
another way to ask, can we say that the protein-DNA is direct binding if I
can show the DNA-protein binding in gel-shift assay?
【在 A******y 的大作中提到】
: http://www.ncbi.nlm.nih.gov/pubmed/15100411
: Not so sure what are you asking, but here is a paper should answer some of
: your questions.
, there is a specific binding site (consensus sequences) on DNA for them to
bind. Without them, no binding. We can use ChIP or EMSA to test it. However,
for histone, there is no specific binding site on DNA. But histone binds to
any DNA. So we call that the histone-DNA binding is specific, but non-
sequence specific.But for some proteins, they may have some kind of
association, but not direct binding with DNA. So my question is, how to
demonstrate that the binding between histone and DNA is a direct binding?
How the binding ability of histone to DNA was found and proved? Or in
another way to ask, can we say that the protein-DNA is direct binding if I
can show the DNA-protein binding in gel-shift assay?
【在 A******y 的大作中提到】
: http://www.ncbi.nlm.nih.gov/pubmed/15100411
: Not so sure what are you asking, but here is a paper should answer some of
: your questions.
A*y
10 楼
Huh? You have x-ray crystal structure showing you that it is binding to the
DNA. The same paper also reconstituted the chromosome using DNA and
histone. In addition, you can see nucleosome with cryo-EM. In real
physical world, binding specificity is achieved through affinity, if you use
enough of DNA and enough random protein, they will bind except it has no
biological significance and just aggregation. To answer your question, even
BSA can shift a DNA gel if you used enough to aggregate the DNA. Shifting
at nano to pico molar range, I call it specific.
DNA. The same paper also reconstituted the chromosome using DNA and
histone. In addition, you can see nucleosome with cryo-EM. In real
physical world, binding specificity is achieved through affinity, if you use
enough of DNA and enough random protein, they will bind except it has no
biological significance and just aggregation. To answer your question, even
BSA can shift a DNA gel if you used enough to aggregate the DNA. Shifting
at nano to pico molar range, I call it specific.
s*i
11 楼
Thermal cooker pot
国内买的,便宜,烧开后焖都不用电,所以我咬咬牙带过来了。
这里的价钱就贵了,两百多一百多最便宜的五十多,你可以到Amazon搜一下。
http://www.huaren.us/dispbbs.asp?boardid=222&id=650808&page=0&star=1
要不你去店里看看慢烧锅slow cooker(Walmart,Target,便宜的二十多三十多),可
以煲汤煮稀饭。
http://www.huaren.us/dispbbs.asp?boardid=222&id=576394&page=0&move=pre
【在 y*****p 的大作中提到】
: 焖烧锅 英文是什么?我也要去买一个。
国内买的,便宜,烧开后焖都不用电,所以我咬咬牙带过来了。
这里的价钱就贵了,两百多一百多最便宜的五十多,你可以到Amazon搜一下。
http://www.huaren.us/dispbbs.asp?boardid=222&id=650808&page=0&star=1
要不你去店里看看慢烧锅slow cooker(Walmart,Target,便宜的二十多三十多),可
以煲汤煮稀饭。
http://www.huaren.us/dispbbs.asp?boardid=222&id=576394&page=0&move=pre
【在 y*****p 的大作中提到】
: 焖烧锅 英文是什么?我也要去买一个。
q*d
12 楼
用慢炖锅好
l*2
13 楼
有学到一样。谢谢啦:)
我以前有个台湾朋友推荐大同电锅,
这个在台湾人眼里简直就是一鍋在手 妙用無窮
是她们出国必备。质量非常好。
我都想买个二手的了。
【在 s*******i 的大作中提到】
: Thermal cooker pot
: 国内买的,便宜,烧开后焖都不用电,所以我咬咬牙带过来了。
: 这里的价钱就贵了,两百多一百多最便宜的五十多,你可以到Amazon搜一下。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=650808&page=0&star=1
: 要不你去店里看看慢烧锅slow cooker(Walmart,Target,便宜的二十多三十多),可
: 以煲汤煮稀饭。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=576394&page=0&move=pre
我以前有个台湾朋友推荐大同电锅,
这个在台湾人眼里简直就是一鍋在手 妙用無窮
是她们出国必备。质量非常好。
我都想买个二手的了。
【在 s*******i 的大作中提到】
: Thermal cooker pot
: 国内买的,便宜,烧开后焖都不用电,所以我咬咬牙带过来了。
: 这里的价钱就贵了,两百多一百多最便宜的五十多,你可以到Amazon搜一下。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=650808&page=0&star=1
: 要不你去店里看看慢烧锅slow cooker(Walmart,Target,便宜的二十多三十多),可
: 以煲汤煮稀饭。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=576394&page=0&move=pre
e*e
18 楼
这个好像有点大,占地方,而且应该挺重的。
【在 s*******i 的大作中提到】
: Thermal cooker pot
: 国内买的,便宜,烧开后焖都不用电,所以我咬咬牙带过来了。
: 这里的价钱就贵了,两百多一百多最便宜的五十多,你可以到Amazon搜一下。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=650808&page=0&star=1
: 要不你去店里看看慢烧锅slow cooker(Walmart,Target,便宜的二十多三十多),可
: 以煲汤煮稀饭。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=576394&page=0&move=pre
【在 s*******i 的大作中提到】
: Thermal cooker pot
: 国内买的,便宜,烧开后焖都不用电,所以我咬咬牙带过来了。
: 这里的价钱就贵了,两百多一百多最便宜的五十多,你可以到Amazon搜一下。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=650808&page=0&star=1
: 要不你去店里看看慢烧锅slow cooker(Walmart,Target,便宜的二十多三十多),可
: 以煲汤煮稀饭。
: http://www.huaren.us/dispbbs.asp?boardid=222&id=576394&page=0&move=pre
e*e
19 楼
上次在target买了一个1.5qt的slow cooker,税前7块钱,两个人喝粥正好,娃哈哈。
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