Tan group striked again... http://www.pnas.org/content/early/2011/02/15/1016197108.abstrac 1. construct a conformation switch-based aptamer sensor which elicits signal upon binding to specific target on cell(have been done many times); 2. use the sensor to quantify cancer cells down to a few hundred cells, in both saline & serum, with a huge dynamic range (new to me, but shouldn't be too hard) 3. use the sensor for in vivo imaging of cancer mouse model induced by SC injected cancer cells. imaging is specific against one type of cancer cells and specific for aptamer sequence. (very similar to the first project I killed... chickened out after a few tries-_-) one major concern is they let the tumor grow to 1-2cm which seems way too big. combining w/ the fact that they killed the mouse b4 imaging (is this the common practice? I know little about imaging), kinda suspect the aptamer wouldn't work in live mice (clearance/ degradation won't allow the aptamer to reach tumor at such high level), and aptamer wouldn't penetrate a more "normal" tumor w/ smaller size and few lesions. still, seems very nice work to me. anyone else care to comment? P.S., is it common to do fluorescence imaging in mice? how "translatable" is it to human, considering all the background and signal penetration issue?
【在 w******e 的大作中提到】 : Tan group striked again... : http://www.pnas.org/content/early/2011/02/15/1016197108.abstrac : 1. construct a conformation switch-based aptamer sensor which elicits : signal upon binding to specific target on cell(have been done many times); : 2. use the sensor to quantify cancer cells down to a few hundred cells, : in both saline & serum, with a huge dynamic range (new to me, but : shouldn't be too hard) : 3. use the sensor for in vivo imaging of cancer mouse model induced by : SC injected cancer cells. imaging is specific against one type of : cancer cells and specific for aptamer sequence.
my point is, w/ a tumor that big, wouldn't there be a lot of necrosis/ lesions going on, which greatly facilitate the tissue penetration, which is THE problem when it comes to in vivo application?
Optical imaging with NIR fluorephore is good for mouse imaging, especially xenograft tumors. For human application, most likely it will be used in optical guided surgery.
x*2
40 楼
那从成都递签到广州的呢?
y*6
41 楼
It seems that Roger Tsien is working on optical guided surgery now.
aptamer against thrombin protein. I guess you are interested in application of aptamer. Lu Yi, Andrew Ellington, Tan weihong, Li Yingfu each published an aptamer-sensor review last year. you can start from there.
In many applications (mouse), NIR optical imaging can substitute PET. Eva M. Sevick's group labeled Transtuzumab with both NIR dye and PET (or SPECT?) agent for mouse tumor imaging. They conclude that NIR optical imaging overperformed PET (or SPECT?) in many aspects. Cannot remember the details. You can find their recent publciations if interested.
b*0
50 楼
再问一句,第一次可以跨区签证吗?
h*o
51 楼
thanks!
【在 w******e 的大作中提到】 : aptamer against thrombin protein. : I guess you are interested in application of aptamer. Lu Yi, Andrew : Ellington, : Tan weihong, Li Yingfu each published an aptamer-sensor review last year. : you can start from there.
n*r
52 楼
除非有很充分的理由,否则风险很大.
【在 b*******0 的大作中提到】 : 再问一句,第一次可以跨区签证吗?
w*e
53 楼
good to know. thx for sharing!
M. .
【在 y****6 的大作中提到】 : In many applications (mouse), NIR optical imaging can substitute PET. Eva M. : Sevick's group labeled Transtuzumab with both NIR dye and PET (or SPECT?) : agent for mouse tumor imaging. They conclude that NIR optical imaging : overperformed PET (or SPECT?) in many aspects. Cannot remember the details. : You can find their recent publciations if interested.