Someone overexpressed a certain microRNA in a cancer cell line and then compared the mRNA profiles of control cells vs microRNA overexpressed cells. It seems very obvious that a bounch of genes from a certain chromsome location were all elevated at mRNA level. Those genes are also functionally related too, but none of them is among the predicted targets of this microRNA according to the results from all the popular bioinformatical web-tools. What could be the mechanism underneath? What should be done to figure out the connection? Your pointers would be highly appreciated.
S*y
6 楼
在monster上upload resume也算吧??
l*e
7 楼
condo or apartment? If they are rental apartment, there is possible safty issue, because maybe some of them are for low income people.
I*t
8 楼
xmarks
a*o
9 楼
This could be due to indirect effect. e.g.these genes are all negatively regulated by a repressor X, X is the target of the microRNA. overexpresseion of the microRNA, downregulated X and derepress the expression of that group of genes.
l*y
10 楼
嗯,谢谢了。 的确有过给我发 email, 要过简历后,就没有下文了 :(
l*7
11 楼
如果是两层以上的房子,你就没隐私了.
h*g
12 楼
新版的firefox 有个syn 的功能
s*6
13 楼
Thanks!! The trouble is that repressor X didn't show itself up in the profiling data, i.e. none of the decreased mRNA seems to be such a repressor and a direct target of that microRNA. No direct "targets" was ever found in the profiling data...but those increased and decreased were indeed confirmed with qPCR. Could microRNAs alter gene expression via a mechanism that is not Waston- Crick base-paring based? Could microRNAs directly interact with protein factors or anything in the transcriptional machine? How to prove or disprove this possibility?
overexpresseion group
【在 a****o 的大作中提到】 : This could be due to indirect effect. e.g.these genes are all negatively : regulated by a repressor X, X is the target of the microRNA. overexpresseion : of the microRNA, downregulated X and derepress the expression of that group : of genes.
It may have additional repressor Y not identified yet.
repressor in
【在 s****6 的大作中提到】 : Thanks!! The trouble is that repressor X didn't show itself up in the : profiling data, i.e. none of the decreased mRNA seems to be such a repressor : and a direct target of that microRNA. No direct "targets" was ever found in : the profiling data...but those increased and decreased were indeed : confirmed with qPCR. : Could microRNAs alter gene expression via a mechanism that is not Waston- : Crick base-paring based? : Could microRNAs directly interact with protein factors or anything in the : transcriptional machine? How to prove or disprove this possibility? :
It has also been reported that certain miRNA binds to 5'UTR or coding seq to activate gene expression (see example: Vasudevan, S., Tong, Y. and Steitz, J.A. (2007). Switching between repression and activation: microRNAs can activate translation. Science. 318: 1931-1934. ). Just throw out possibilities.