genotyping 问题求助 - 未名空间MITBBS历史存档

国际科技财经博客移民网络热点娱乐民生时事公众号

Redian新闻

>未名空间

>Biology - 生物学

genotyping 问题求助

genotyping 问题求助# Biology - 生物学

o*e2011-04-13 07:04

1 楼

【以下文字转载自 Seattle 讨论区】
发信人: onetiemyshoe (onetiemyshoe), 信区: Seattle
标题: 微软：撒剔牙的Karma屠刀不会休息
发信站: BBS 未名空间站 (Thu Oct 30 10:24:18 2014, 美东)
18000karma based layoff.
明年继续。撒剔牙的karma屠刀
不会休息的。
为什么？这个文章可以有启示：
https://beta.cironline.org/reports/job-brokers-exploit-indian-tech-workers-
on-taxpayers-dime/
800,000 H1b worker 现在美国，
大半是烙印奴工。很多烙印奴工有
很长时间拿不到合同。所以经济
驱使，烙印猎头-资本家-管理-技术人员
必须联合一起黑老中老美竞争对手，职场掐死老中
老美STEM毕业生。
居然还有老中老美一厢情愿地”联合“共同价值的烙印
。。。

H*i2011-04-13 07:04

2 楼

但是古人云：行百里者半九十。我们离终点还很远啊！
我自己正在通过朋友游说参议员Jeff Merkley去co sponsor S.1857
-----------------------------------------------------------
立法十步路：
第一步立草案；
第二步委员会初审；
第三步分配给小组委员会听证；
第四步小组委员会修改上报委员会；
第五步委员会终审上报；
第六步参院或者众院表决
----------------------我们已经走到这里了---------------
第七步众院或者参院表决（第六步是众院第七步就是参院，第六步是参院第七步就是众
院）
第八步两院通过修正案
第九步总统签署（总统可以否决或者签署或者置之不理，如果十天置之不理但是两院正
常工作，修正案自动成为法案，如果十天置之不理而且两院已经开始第二次休会，修正
案死亡）
第十步推翻总统否决（如果总统否决，两院都要2/3票才能推翻总统否决）
-------------------------------------------------------
虽然看起来只有十步路，但是实际上是十四步路，因为第七步路要在另一个院重走第二
三四五步路，所以革命尚未成功，同学仍须努力！
-------------------------------------------------------
大家去facebook like Mike Lee吧！他是senate bill sponsor!
大家如果不知道写什么就copy and paste 下面这句话：
Thank you Mike for sponsoring S.1857!!! Congratulations on H.R.3012 passing
the House!
-------------谢谢谢谢谢谢谢谢谢谢谢谢谢谢--------------
请大家到自己的参议员甚至别的州的参议员facebook网页上去留言，模版如下：
Dear Senator XXXX XXXX,
Please co-sponsor Mike Lee's S.1857, that'll help a lot of people and help U.S. in the long run. We'll remember your help and vote for you!
http://thomas.loc.gov/cgi-bin/bdquery/z?d112:SN01857:
This is a very small bill with only a few items. The related H.R.3012 just passed the House with 389 vs. 15. S.1857 is almost exactly the same as H.R.3012.
Thanks a lot.
-------------谢谢谢谢谢谢谢谢谢谢谢谢谢谢--------------
请大家到舒默网页支持：
http://www.facebook.com/chuckschumer
Dear Senator Schumer,
Thank you for planning to facilitate the bill to end country limits for
worker visas, that'll help a lot of people and help U.S. in the long run. We
'll remember your help and vote for you!
http://www.boston.com/news/nation/washington/articles/2011/11/2
http://thomas.loc.gov/cgi-bin/bdquery/z?d112:SN01857:
Thanks a lot.
-------------谢谢谢谢谢谢谢谢谢谢谢谢谢谢--------------
How a Bill Becomes Law?
There are potentially 10 steps a bill can go through before becoming a law.
Below is a description of each step in the process, using the Genetic
Information Non-Discrimination Act of 2003 (S. 1053), as an example.
Step 1: A Bill Is Born
Anyone may draft a bill; however, only members of Congress can introduce
legislation, and, by doing so, become the sponsor(s). The president, a
member of the cabinet or the head of a federal agency can also propose
legislation, although a member of Congress must introduce it.
Step 2: Committee Action
As soon as a bill is introduced, it is referred to a committee. At this
point the bill is examined carefully and its chances for passage are first
determined. If the committee does not act on a bill, the bill is effectively
"dead."
Step 3: Subcommittee Review
Often, bills are referred to a subcommittee for study and hearings. Hearings
provide the opportunity to put on the record the views of the executive
branch, experts, other public officials and supporters, and opponents of the
legislation.
Step 4: Mark up
When the hearings are completed, the subcommittee may meet to "mark up" the
bill; that is, make changes and amendments prior to recommending the bill to
the full committee. If a subcommittee votes not to report legislation to
the full committee, the bill dies. If the committee votes for the bill, it
is sent to the floor.
Step 5: Committee Action to Report a Bill
After receiving a subcommittee's report on a bill the full committee votes
on its recommendation to the House or Senate. This procedure is called "
ordering a bill reported."
Step 6: Voting
After the debate and the approval of any amendments, the bill is passed or
defeated by the members voting.
---------we are here now-------there's a long way to go-----------
Step 7: Referral to Other Chamber
When the House or Senate passes a bill, it is referred to the other chamber,
where it usually follows the same route through committee and floor action.
This chamber may approve the bill as received, reject it, ignore it, or
change it.
Step 8: Conference Committee Action
When the actions of the other chamber significantly alter the bill, a
conference committee is formed to reconcile the differences between the
House and Senate versions. If the conferees are unable to reach agreement,
the legislation dies. If agreement is reached, a conference report is
prepared describing the committee members' recommendations for changes. Both
the House and Senate must approve the conference report.
Step 9: Final Action
After both the House and Senate have approved a bill in identical form, it
is sent to the president. If the president approves of the legislation, he
signs it and it becomes law. Or, if the president takes no action for ten
days, while Congress is in session, it automatically becomes law. If the
president opposes the bill he can veto it; or if he takes no action after
the Congress has adjourned its second session, it is a "pocket veto" and the
legislation dies.
Step 10: Overriding a Veto
If the president vetoes a bill, Congress may attempt to "override the veto."
If both the Senate and the House pass the bill by a two-thirds majority,
the president's veto is overruled and the bill becomes a law.
-----谢谢谢谢谢谢谢谢谢谢谢谢谢谢谢谢谢谢------
H.R.3012
Latest Title: Fairness for High-Skilled Immigrants Act
Sponsor: Rep Chaffetz, Jason [UT-3] (introduced 9/22/2011)
Related Bills: S.1857
Latest Major Action: 11/18/2011 Placed on the Union Calendar, Calendar No.
193.
House Reports: 112-292
--------------------------------------------
Cosponsors (11)
Rep Flake, Jeff [AZ-6] - 11/18/2011
Rep Goodlatte, Bob [VA-6] - 11/4/2011
Rep Griffin, Tim [AR-2] - 10/11/2011
Rep Gutierrez, Luis V. [IL-4] - 11/4/2011
Rep Holt, Rush D. [NJ-12] - 10/31/2011
Rep Jackson, Jesse L., Jr. [IL-2] - 11/15/2011
Rep Lofgren, Zoe [CA-16] - 10/25/2011
Rep Maloney, Carolyn B. [NY-14] - 11/18/2011
Rep Moran, James P. [VA-8] - 11/18/2011
Rep Smith, Lamar [TX-21] - 9/22/2011
Rep Thompson, Glenn [PA-5] - 11/16/2011

t*n2011-04-13 07:04

3 楼

鼓起小小的勇气上来贴一首自己很喜欢但是唱不好的歌 :(
建议大家一开始音量开小一些，刚进声音的时候人声很唐突。
过会儿再上来贴首正经点的歌哈~
词曲：Norah Jones
原唱：Norah Jones
What am I to you
Tell me darling true
To me you are the sea
Fast as you can be
And deep the shade of blue
When you're feeling low
To whom else do you go
See I cry if you hurt
I'd give you my last shirt
Because I love you so
If my sky should fall
Would you even call
Opened up my heart
I never want to part
I'm giving you the ball
When I look in your eyes
I can feel the butterflies
I love you when you're blue
Tell me darlin' true
What am I to you

S*e2011-04-13 07:04

4 楼

你们用的Hadoop Cluster是怎么升级的？下面是我的问题
Rolling Upgrade Hadoop Cluster Question
In our company, one of main Hadoop clusters (HDP) has about 600 nodes. It
upgrades almost monthly plus some other maintenance. Every time doing so
takes hours to a couple of days and all apps running on it have to be shut
off. I just cannot imagine the clusters performing such important work in
other companies will get interrupted so often and so long. I asked why don't
we do rolling upgrade? Here is one of main architect's answer. Is it true?
How about the upgrades in your company?
================================================
Regarding rolling upgrades, I want to be careful that everyone
understand what happens during this process. Up to 12 nodes per hour get
upgraded to the next version of HDP. As this process continues with each
passing hour the capacity of the cluster is reduced by X number of nodes
that get completed. When the cluster gets in the neighborhood of 75% a
restart is required for most of the services. The core services are handled
under the up-time such as MapReduce, HDFS, Name Node HA, Resource Manager HA
, Zookeeper and Hive HA if it is configured. Spark, Kafka, Storm and the
other services are not included in Rolling upgrade with no downtime. Express
upgrade has allowed our team to upgrade the clusters in a much faster
timeframe. The last upgrade of the cluster was 5 hours. I believe the issue
of downtime you stated above with 2 days and 4 hours would not be correct
for the actual HDP downtime. This is likely the entire maintenance which
would include Ambari Upgrades, HDP upgrades, stopping jobs, sanity checks,
and restarting all of the jobs to complete catch up with batch processing. I
would like to suggest that your team is engaged with the messages that will
be sent out and stop your job at the time the upgrade will be executing
which would be on Saturday morning. When the upgrade is completed you will
be able to start your job again, another notification will be sent out.

z*u2011-04-13 07:04

5 楼

刚进了个TetO-H2BGFP transgenic 耗子。文献里面给出的primer不能检测difference b/w
homo- or heterogenic transgenic alleles. 咋办？

o*e2011-04-13 07:04

6 楼

w*t2011-04-13 07:04

7 楼

赶快参院桑拿喽..然后出与列...

g*y2011-04-13 07:04

8 楼

沙发，竟然高糖没坐

S*s2011-04-13 07:04

9 楼

rolling upgrades会有很多data reblancing带来的系统负担
如果本来系统就很忙了，那是会出问题的
做好先把富余搞大一点，这样滚动升级带来的额外负担不影响已有的工作负荷

't
?

【在 S*******e 的大作中提到】

: 你们用的Hadoop Cluster是怎么升级的？下面是我的问题
: Rolling Upgrade Hadoop Cluster Question
: In our company, one of main Hadoop clusters (HDP) has about 600 nodes. It
: upgrades almost monthly plus some other maintenance. Every time doing so
: takes hours to a couple of days and all apps running on it have to be shut
: off. I just cannot imagine the clusters performing such important work in
: other companies will get interrupted so often and so long. I asked why don't
: we do rolling upgrade? Here is one of main architect's answer. Is it true?
: How about the upgrades in your company?
: ================================================

l*g2011-04-13 07:04

10 楼

需要用real time PCR

/w

【在 z****u 的大作中提到】

: 刚进了个TetO-H2BGFP transgenic 耗子。文献里面给出的primer不能检测difference b/w
: homo- or heterogenic transgenic alleles. 咋办？

o*e2011-04-13 07:04

11 楼

顶

j*w2011-04-13 07:04

12 楼

这样一过，是不是对1月排期应该是利好？

【在 w*******t 的大作中提到】

: 赶快参院桑拿喽..然后出与列...

b*p2011-04-13 07:04

13 楼

bdbd

w*g2011-04-13 07:04

14 楼

0.21.0用到今天的飘过。

't
?

【在 S*******e 的大作中提到】

z*u2011-04-13 07:04

15 楼

可行吗？因为理论上讲homo- and heterogenic transgenic 只差一个copy, 那在real
time pcr 上面只会反映出critical cycle number difference by one. 这可不太准确
阿。

【在 l******g 的大作中提到】

: 需要用real time PCR
:
: /w

y*n2011-04-13 07:04

16 楼

orthogonal

【在 j******w 的大作中提到】

: 这样一过，是不是对1月排期应该是利好？

i*i2011-04-13 07:04

17 楼

lol。。。
火鸡林唱得真好！

【在 g**********y 的大作中提到】

: 沙发，竟然高糖没坐

a*s2011-04-13 07:04

18 楼

一般公司都是物尽其用，大cluster意味着运行的程序更多。要找富余，只有根据历史
记录找application少的时间。这段时间能否保证所有都upgrade。不清楚是不是
hortonworks自己做过大规模RU测试没有。

【在 S***s 的大作中提到】

: rolling upgrades会有很多data reblancing带来的系统负担
: 如果本来系统就很忙了，那是会出问题的
: 做好先把富余搞大一点，这样滚动升级带来的额外负担不影响已有的工作负荷
:
: 't
: ?

h*n2011-04-13 07:04

19 楼

get sequence of the chromasome of the transgene and design new primers

w*t2011-04-13 07:04

20 楼

理工的...

【在 y***n 的大作中提到】

: orthogonal

b*p2011-04-13 07:04

21 楼

好听！跟mm之前的歌又是不一样的风格，厉害啊~
今天晚上流行英文歌呀~

z*u2011-04-13 07:04

22 楼

这才是难点阿。构建这个strain的人都不知道transgene的位置。
能否用reverse PCR通过construct向外扩增出两端的序列。没做过这种pcr

【在 h********n 的大作中提到】

: get sequence of the chromasome of the transgene and design new primers

H*i2011-04-13 07:04

23 楼

有可能的。奥本作为专家证人曾经被两院委员会和小组委员会咨询，奥本是支持这个法
案的。

【在 j******w 的大作中提到】

: 这样一过，是不是对1月排期应该是利好？

j*t2011-04-13 07:04

24 楼

哇，好听！

【在 t*******n 的大作中提到】

: 鼓起小小的勇气上来贴一首自己很喜欢但是唱不好的歌 :(
: 建议大家一开始音量开小一些，刚进声音的时候人声很唐突。
: 过会儿再上来贴首正经点的歌哈~
: 词曲：Norah Jones
: 原唱：Norah Jones
: What am I to you
: Tell me darling true
: To me you are the sea
: Fast as you can be
: And deep the shade of blue

l*g2011-04-13 07:04

25 楼

我们就是用这方法鉴定transgene的copy number.
随笔google了一下,这地方也用:
http://catalog.takara-bio.co.jp/en/product/tech_info_detail2.as

real

【在 z****u 的大作中提到】

: 可行吗？因为理论上讲homo- and heterogenic transgenic 只差一个copy, 那在real
: time pcr 上面只会反映出critical cycle number difference by one. 这可不太准确
: 阿。

H*i2011-04-13 07:04

26 楼

有可能的。奥本作为专家证人曾经被两院委员会和小组委员会咨询，奥本是支持这个法
案的。

【在 j******w 的大作中提到】

: 这样一过，是不是对1月排期应该是利好？

h*72011-04-13 07:04

27 楼

初听的时候还以为是原唱呢，入场时候很惊艳，英语发音也很好，你平时说英语怎么样
？

z*u2011-04-13 07:04

28 楼

awesome!多谢了！

【在 l******g 的大作中提到】

: 我们就是用这方法鉴定transgene的copy number.
: 随笔google了一下,这地方也用:
: http://catalog.takara-bio.co.jp/en/product/tech_info_detail2.as
:
: real

l*i2011-04-13 07:04

29 楼

奥本先行一步好了

【在 H******i 的大作中提到】

: 有可能的。奥本作为专家证人曾经被两院委员会和小组委员会咨询，奥本是支持这个法
: 案的。

k*i2011-04-13 07:04

30 楼

这明明比Norah Jones唱的好听啊。。

【在 t*******n 的大作中提到】

f*c2011-04-13 07:04

31 楼

“虽然看起来只有10步路，但是实际上是14步路，因为第7步路要在另一个院重走第二
三四五步路，所以革命尚未成功，同学仍须努力！”
这个第7步里的，二三四五步，都会啥时候开始？还是参院可能置之不理？

【在 H******i 的大作中提到】

: 但是古人云：行百里者半九十。我们离终点还很远啊！
: 我自己正在通过朋友游说参议员Jeff Merkley去co sponsor S.1857
: -----------------------------------------------------------
: 立法十步路：
: 第一步立草案；
: 第二步委员会初审；
: 第三步分配给小组委员会听证；
: 第四步小组委员会修改上报委员会；
: 第五步委员会终审上报；
: 第六步参院或者众院表决

t*n2011-04-13 07:04

32 楼

说英语还不错哦，不过不常说就是了。。。。闷头做research，呵呵

初听的时候还以为是原唱呢，入场时候很惊艳，英语发音也很好，你平时说英语怎么样？
★ Sent from iPhone App: iReader Mitbbs 7.52 - iPad Lite

【在 h*********7 的大作中提到】

: 初听的时候还以为是原唱呢，入场时候很惊艳，英语发音也很好，你平时说英语怎么样
: ？

t*n2011-04-13 07:04

33 楼

害羞了害羞了！

这明明比Norah Jones唱的好听啊。。
★ Sent from iPhone App: iReader Mitbbs 7.52 - iPad Lite

【在 k****i 的大作中提到】

: 这明明比Norah Jones唱的好听啊。。