M*n
2 楼
FACS能不能sort aggregates of cells?
不是single cell, FACS 机器能行吗?
请各位有经验的指教。谢谢。
不是single cell, FACS 机器能行吗?
请各位有经验的指教。谢谢。
p*s
3 楼
如果有谁家老人和我一趟飞机
我可以帮忙
我可以帮忙
a*d
4 楼
depends on the size of cell aggregates. dual cells or triple cells are fine
for cell sorting. But big cell aggregates can't pass through and may get
machine jammed.
for cell sorting. But big cell aggregates can't pass through and may get
machine jammed.
M*n
6 楼
i found online that the lysate needs to be pre-filtered with 70um mesh
before sorting.
if the aggregates are smaller than 70um (say 60um), is it possible to sort?
fine
【在 a****d 的大作中提到】
: depends on the size of cell aggregates. dual cells or triple cells are fine
: for cell sorting. But big cell aggregates can't pass through and may get
: machine jammed.
before sorting.
if the aggregates are smaller than 70um (say 60um), is it possible to sort?
fine
【在 a****d 的大作中提到】
: depends on the size of cell aggregates. dual cells or triple cells are fine
: for cell sorting. But big cell aggregates can't pass through and may get
: machine jammed.
a*d
8 楼
I do use 45um filter to filter through the lysate before preparing the rest
procedures. I guess you can collect smaller aggregates less than certain
diameters.
The other thing is the purity of your cells. If the lysate is prepared from
heterogeneous population, where cells have different sizes, than you have to
be careful to isolate large cells and small aggregates.
【在 M*****n 的大作中提到】
: i found online that the lysate needs to be pre-filtered with 70um mesh
: before sorting.
: if the aggregates are smaller than 70um (say 60um), is it possible to sort?
:
: fine
procedures. I guess you can collect smaller aggregates less than certain
diameters.
The other thing is the purity of your cells. If the lysate is prepared from
heterogeneous population, where cells have different sizes, than you have to
be careful to isolate large cells and small aggregates.
【在 M*****n 的大作中提到】
: i found online that the lysate needs to be pre-filtered with 70um mesh
: before sorting.
: if the aggregates are smaller than 70um (say 60um), is it possible to sort?
:
: fine
W*a
9 楼
你要tweak FACS才能收集aggregate,因为它default是要把所有不是single cell
event都排除掉的。此外cell aggregate形状不规则,你很难收集一个homogenous的
sample,会有很大的样本偏差。如果你规定只收集2 cell的,倒是还可能比较纯
sort?
【在 M*****n 的大作中提到】
: i found online that the lysate needs to be pre-filtered with 70um mesh
: before sorting.
: if the aggregates are smaller than 70um (say 60um), is it possible to sort?
:
: fine
event都排除掉的。此外cell aggregate形状不规则,你很难收集一个homogenous的
sample,会有很大的样本偏差。如果你规定只收集2 cell的,倒是还可能比较纯
sort?
【在 M*****n 的大作中提到】
: i found online that the lysate needs to be pre-filtered with 70um mesh
: before sorting.
: if the aggregates are smaller than 70um (say 60um), is it possible to sort?
:
: fine
d*p
11 楼
100um nozzle works better for bigger cells or aggregates. You may simply
gate a region with the size you need. Size and shape are not the same, same
shape must have same size, or similar size is more likely to have better
uniform in shape.
gate a region with the size you need. Size and shape are not the same, same
shape must have same size, or similar size is more likely to have better
uniform in shape.
相关阅读
如果当research associate 能挣个5,6 万是不是大家也就不折腾了paper help please. Thanks.请教: T-test VS ANOVA请教:应该是类似蛋白溶解和吸附方面的问题?请教做long bone section 的高手请问前辈,工业界是不是没有绿卡就没可能?我当年梦想的两大发明都还没有实现肿瘤免疫这个领域怎么样?什么方法能买到处方药用于小鼠试验?Paper Help国内生物PHD不需要本科毕业即可读请教提取细胞核蛋白后WB背景高的问题14位博士后北京怀柔挂职副处请教一下微量RNA用什么方法测定比较好生物版内可否再开一个子板作为纯粹的学术版统计盲人就指点审稿机会co-first和second author怎么安排的?Paper Help!! From Advances in Chronic Kidney Disease如何寻找某个基因的Transcription factor?