L*o
2 楼
EphrinB2/EphB4 ? or COUP-TFII?
n*w
3 楼
neuropilin 1 and 2?
z*u
4 楼
楼上俩能不能给推荐个哪个公司的Ab啊,COUP-TFII, Neurophilin1,2。。mouse的
tissue. EphrinB2,4 我已经放弃了,试了俩公司的都不行。
tissue. EphrinB2,4 我已经放弃了,试了俩公司的都不行。
n*w
5 楼
http://www.ncbi.nlm.nih.gov/pubmed/21852397
you can look it up.
I don't know what kind of tissue you are staining.
For retina, you can tell arteries and veins simply by their morphology. I
assume this may apply to other tissues.
you can look it up.
I don't know what kind of tissue you are staining.
For retina, you can tell arteries and veins simply by their morphology. I
assume this may apply to other tissues.
j*d
6 楼
How to tell?
【在 n***w 的大作中提到】
: http://www.ncbi.nlm.nih.gov/pubmed/21852397
: you can look it up.
: I don't know what kind of tissue you are staining.
: For retina, you can tell arteries and veins simply by their morphology. I
: assume this may apply to other tissues.
C*S
9 楼
问一个问题:
这些个动脉静脉marker,在capillary endothelial cell上表达情况怎么样?有没有
capillary endothelial cell specific marker?
surrounded
【在 n***w 的大作中提到】
: if you look at figures in that paper I mentioned,
: if you have a whole mount of retina stained by IB4 or other markers,
: you can tell that arteries are clear from others while veins are surrounded
: by capillaries.
这些个动脉静脉marker,在capillary endothelial cell上表达情况怎么样?有没有
capillary endothelial cell specific marker?
surrounded
【在 n***w 的大作中提到】
: if you look at figures in that paper I mentioned,
: if you have a whole mount of retina stained by IB4 or other markers,
: you can tell that arteries are clear from others while veins are surrounded
: by capillaries.
z*u
10 楼
多谢楼上各位意见。 我可能会尝试COUP-TFII。因为samople是H2B-GFP表达老鼠,需要
GFP+arteriole marker. ephrinB2 staining如果过太复杂或者涉及antigen retrieval
,GFP可能会受影响。
另外我找到一个smoothelin 文献说特异表达在arteriole的SMC里,而不在venule的SMC
.
形态学上动脉和静脉还是很好区分的。但是我想最好还是用多个marker以及形态学一起
来验证我的GFP-expressing cell的位置。所以才在寻找这些marker
GFP+arteriole marker. ephrinB2 staining如果过太复杂或者涉及antigen retrieval
,GFP可能会受影响。
另外我找到一个smoothelin 文献说特异表达在arteriole的SMC里,而不在venule的SMC
.
形态学上动脉和静脉还是很好区分的。但是我想最好还是用多个marker以及形态学一起
来验证我的GFP-expressing cell的位置。所以才在寻找这些marker
相关阅读
RNA-seq input的问题请教有没有一种研究名字叫case-case control生物、化学、物理成功转CS的不要太多问个RNAseq问题histone western blot 求教问一下做chip-seq的版友----Paper help!-----静脉曲张 干细胞大量内荐湾区各级软件工程师和QA,含entry level学R的书生物类企业的博士后怎么样,有过来人么?新杂志 J Biol Methods, 请捧场Molecular cell 如果文章送审了会通知吗问下有人参加UC Davis Bioinformatics Training course吗Cd8+T cell depltion by anti-cd8正在写review,问个问题这个太牛了生物你能不能走慢点 (转载)一个癌症sample病理学问题好神奇的鱼