T*9
2 楼
Got approval notice at 11 pm today.
NIW E-File TSC
140 RD 07/25/2007
PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
materials arrived on August)
485 RD 8/16/2007
RFE in 2009 for job verification.
Be patient, you will get approved soon. Looks like the approval is totally
random.
Good luck to all.
NIW E-File TSC
140 RD 07/25/2007
PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
materials arrived on August)
485 RD 8/16/2007
RFE in 2009 for job verification.
Be patient, you will get approved soon. Looks like the approval is totally
random.
Good luck to all.
b*s
3 楼
I tried to amply 2XGFP by one PCR and subclone it into some vectors. The
template contains two tandem GFPs but PCR can produce only one 700bp band.
The 1.4K band is very weak and smeared. Before redesigning long gateway
primers to include a little flanking regions, I want to see if anybody have
trick for such PCR? Thanks a lot!
template contains two tandem GFPs but PCR can produce only one 700bp band.
The 1.4K band is very weak and smeared. Before redesigning long gateway
primers to include a little flanking regions, I want to see if anybody have
trick for such PCR? Thanks a lot!
u*n
4 楼
吃
x*n
5 楼
可以用同尾酶分两次加入
ek
6 楼
chi
a*d
7 楼
Cong!
c*9
8 楼
gxgx
d*n
9 楼
gxgx, baozi!
k*s
10 楼
gxgx
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
c*s
11 楼
gx
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
k*o
12 楼
Cong!
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
g*g
13 楼
看来快批完大潮的了
f*u
14 楼
恭喜
c*r
15 楼
gongxi
t*9
16 楼
cong.
Baozi
thanks.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
Baozi
thanks.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
C*X
17 楼
Cong
l*o
18 楼
gx
b*i
19 楼
gxgx!
baozi!
baozi!
c*y
20 楼
gx
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
z*o
21 楼
gxgx!!!
baozi pls
baozi pls
j*4
22 楼
gxgx
m*g
23 楼
gxgx!
y*z
24 楼
gxgx, chi
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
c*4
25 楼
gxgx
a*e
26 楼
恭喜
★ 发自iPhone App: ChineseWeb - 中文网站浏览器
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
★ 发自iPhone App: ChineseWeb - 中文网站浏览器
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
H*e
27 楼
cong
k*o
28 楼
gx
W*t
29 楼
Dude, congratulations!
n*s
30 楼
Congrats! baozi
Some greens today.
Some greens today.
r*3
31 楼
恭喜!
t*o
32 楼
恭喜!
baozi!
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
baozi!
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
m*l
33 楼
gxgx
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
m*e
34 楼
GX~~
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
f*y
35 楼
恭喜,排!
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
a*e
36 楼
cong!
c*n
37 楼
cong
d*n
38 楼
gxgx, baozi!
f*c
39 楼
congs
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
B*o
40 楼
Cong!
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
c*i
41 楼
chi
a*n
42 楼
Congratulations.
l*e
43 楼
gx
h*8
44 楼
congrats!
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
a*n
45 楼
恭喜恭喜!
也沾点喜气。。。
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
也沾点喜气。。。
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
b*n
46 楼
cong
g*5
47 楼
gxgx
s*2
48 楼
gxgx
f*i
49 楼
cong!
r*0
50 楼
gxgx
p*l
51 楼
gxgx!
l*y
52 楼
Con!
f*3
53 楼
gxgx
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
s*7
54 楼
吃包子~~~
s*a
55 楼
cong
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
【在 T****9 的大作中提到】
: Got approval notice at 11 pm today.
: NIW E-File TSC
: 140 RD 07/25/2007
: PD 8/6/2007 (never understood why my PD is on August, maybe my supporting
: materials arrived on August)
: 485 RD 8/16/2007
: RFE in 2009 for job verification.
: Be patient, you will get approved soon. Looks like the approval is totally
: random.
: Good luck to all.
i*n
56 楼
Cong
b*i
57 楼
GXGX
k*e
58 楼
GX
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