C*S
2 楼
先做个mini prep,看看产率怎么样
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
g*j
3 楼
本来要提的就不多
干脆算了，今天重接种算了
【在 C**S 的大作中提到】
: 先做个mini prep,看看产率怎么样
干脆算了，今天重接种算了
【在 C**S 的大作中提到】
: 先做个mini prep,看看产率怎么样
m*M
4 楼
I specially tested overnight e coli cultures harboring plasmid with and
without antibotics, and turned out that I even got more plasmids than those
with antibotics. Try to isolate them (one or two if you don't want to waste
your miniprep column), and tell us your results.
without antibotics, and turned out that I even got more plasmids than those
with antibotics. Try to isolate them (one or two if you don't want to waste
your miniprep column), and tell us your results.
g*j
5 楼
如果等会儿有时间就提一下，不过以前没提过这个质粒，所以产率没法儿比较
those
waste
【在 m****M 的大作中提到】
: I specially tested overnight e coli cultures harboring plasmid with and
: without antibotics, and turned out that I even got more plasmids than those
: with antibotics. Try to isolate them (one or two if you don't want to waste
: your miniprep column), and tell us your results.
those
waste
【在 m****M 的大作中提到】
: I specially tested overnight e coli cultures harboring plasmid with and
: without antibotics, and turned out that I even got more plasmids than those
: with antibotics. Try to isolate them (one or two if you don't want to waste
: your miniprep column), and tell us your results.
s*9
6 楼
还是不要提了，重新接种，重新摇，别忘了加抗生素。
g*5
7 楼
my labmate asked plasmid from me and put it directly in LB, and she tried 3
times, no cloudy and asked me why....
times, no cloudy and asked me why....
g*j
8 楼
不至于吧，总得有点儿啊
另外时间不多，就没提
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
另外时间不多，就没提
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
s*s
9 楼
没戏
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
t*m
10 楼
lol~
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
M*n
11 楼
start over.
it will save you time.
it will save you time.
s*g
12 楼
不！
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
w*n
13 楼
谁这么有才把他弄进来的？
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
3
【在 g*********5 的大作中提到】
: my labmate asked plasmid from me and put it directly in LB, and she tried 3
: times, no cloudy and asked me why....
w*g
14 楼
Kao, you are biological WSN
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
【在 g***j 的大作中提到】
: 昨天接种的时候忘记加抗生素了，质粒提还是不提啊
l*2
15 楼
我干过，没事。。。
g*j
16 楼
哈哈，我才反应过来
【在 w******n 的大作中提到】
: 谁这么有才把他弄进来的？
:
: 3
【在 w******n 的大作中提到】
: 谁这么有才把他弄进来的？
:
: 3
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