求junctional proteins （ZO-1, V-Cadherin, etc）抗体～谢谢～ - 未名空间MITBBS历史存档

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求junctional proteins （ZO-1, V-Cadherin, etc）抗体～谢谢～

求junctional proteins （ZO-1, V-Cadherin, etc）抗体～谢谢～# Biology - 生物学

L*r2012-03-19 07:03

1 楼

只扫了自己的号前后左右一些的，感谢leftHand大侠的code。
"1290147243 -I131, AP:Initial Review "
"1290147245 -I131, AP:Card/ Document Production "
"1290147266 -I765, EAD:Card/ Document Production "
"1290147312 -I765, EAD:Card/ Document Production "
"1290147319 -I485, GC:Initial Review "
"1290147320 -I131, AP:Initial Review "
"1290147324 -I485, GC:Initial Review "
"1290147325 -I131, AP:Initial Review "
"1290147350 -I765, EAD:Initial Review "
"1290147370 -I131, AP:Post Decision Activity "
"1290147438 -I765, EAD:Card/ Document Production "
"1290147464 -I765, EAD:Card/ Document Production "
"1290147465 -I765, EAD:Card/ Document Production "
"1290147469 -I765, EAD:Card/ Document Production "
"1290147479 -I765, EAD:Card/ Document Production "
"1290147483 -I765, EAD:Initial Review "
"1290147547 -I131, AP:Post Decision Activity "
"1290147568 -I765, EAD:Card/ Document Production "
"1290147596 -I765, EAD:Initial Review "
"1290147598 -I131, AP:Initial Review "
"1290147612 -I765, EAD:Card/ Document Production "
"1290147639 -I765, EAD:Card/ Document Production "
"1290147654 -I765, EAD:Card/ Document Production "
"1290147657 -I765, EAD:Card/ Document Production "
"1290147661 -I765, EAD:Initial Review "
"1290147664 -I765, EAD:Card/ Document Production "
"1290147668 -I765, EAD:Card/ Document Production "
"1290147669 -I765, EAD:Card/ Document Production "
"1290147690 -I765, EAD:Card/ Document Production "
"1290147750 -I765, EAD:Initial Review "
"1290147772 -I765, EAD:Card/ Document Production "
"1290147786 -I765, EAD:Card/ Document Production "

n*w2012-03-19 07:03

2 楼

最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。
试了几个zymed (现invitrogen的)，和DSHB的ZO-1，但是都不work。Western and IHC
都不行。
版上有人做过吗？
有些经验trick啥的吗？可以推荐下好用的抗体吗？谢谢

S*n2012-03-19 07:03

3 楼

请问这个区间RD是多少？THX！！

s*y2012-03-19 07:03

4 楼

Sigma 的monoclonal anti-PAN cadherin (cat# C1821) 还不错。
对VE-cadherin 肯定可以。我前几个星期刚在内皮细胞里试过。
注意要买这个单抗的。他们的那个多抗的质量太不稳定了，可把我坑苦了！
前一阵子还上来诉苦的。

IHC

【在 n***w 的大作中提到】

: 最近在试一些junctional proteins, 如ZO-1, VE-Cadherin等等。。。
: 试了几个zymed (现invitrogen的)，和DSHB的ZO-1，但是都不work。Western and IHC
: 都不行。
: 版上有人做过吗？
: 有些经验trick啥的吗？可以推荐下好用的抗体吗？谢谢

p*e2012-03-19 07:03

5 楼

Zo1我们用invitrogen Mouse的，还不错，但是兔子的不work.

IHC

【在 n***w 的大作中提到】

n*w2012-03-19 07:03

6 楼

前面没说清楚，
我就是要染老鼠tissue，所以我尽量避免mouse mAb...

【在 p*****e 的大作中提到】

: Zo1我们用invitrogen Mouse的，还不错，但是兔子的不work.
:
: IHC

n*w2012-03-19 07:03

7 楼

谢谢嗯，我是想染whole retina嗯，所以避免单抗。。

【在 s******y 的大作中提到】

: Sigma 的monoclonal anti-PAN cadherin (cat# C1821) 还不错。
: 对VE-cadherin 肯定可以。我前几个星期刚在内皮细胞里试过。
: 注意要买这个单抗的。他们的那个多抗的质量太不稳定了，可把我坑苦了！
: 前一阵子还上来诉苦的。
:
: IHC

s*y2012-03-19 07:03

8 楼

oh....那就没办法了。。。
Sigma 也有Rabbit anti PAN cadherin，但是是个多抗，每一批都不一样！
倒是每次都能做WB 是没有问题的，染经典的E-cadherin 也每批都行，
就是染N, VE 不是每批都行

【在 n***w 的大作中提到】

: 前面没说清楚，
: 我就是要染老鼠tissue，所以我尽量避免mouse mAb...

p*e2012-03-19 07:03

9 楼

那就不知道了，不好意思，我也是初学者

【在 n***w 的大作中提到】

: 前面没说清楚，
: 我就是要染老鼠tissue，所以我尽量避免mouse mAb...

g*y2012-03-19 07:03

10 楼

我们用很多mouse antibody 染老鼠tissue...

【在 n***w 的大作中提到】

: 前面没说清楚，
: 我就是要染老鼠tissue，所以我尽量避免mouse mAb...

n*w2012-03-19 07:03

11 楼

嗯，你们是怎么处理的？因为我要染血管，所以比较担心。。。。
用no primary做对照？还是用了什么特别的kit，比如说vector labs的m.o.m？
谢谢。
真希望有些GFP junctional protein mice。

【在 g***y 的大作中提到】

: 我们用很多mouse antibody 染老鼠tissue...

g*y2012-03-19 07:03

12 楼

we stain brain tissues.we perfuse mouse first
yes

【在 n***w 的大作中提到】

: 嗯，你们是怎么处理的？因为我要染血管，所以比较担心。。。。
: 用no primary做对照？还是用了什么特别的kit，比如说vector labs的m.o.m？
: 谢谢。
: 真希望有些GFP junctional protein mice。

l*12012-03-19 07:03

13 楼

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【在 n***w 的大作中提到】

: 谢谢嗯，我是想染whole retina嗯，所以避免单抗。。