l*r
2 楼
本届晚会里最好听的一首了
H*e
3 楼
Hi experts,
I need to sequence PI3K and PTEN in tumors for determine whether there is
mutation. But I do not know much about sequencing mutations since I only
work kinase for years. So I have a few basic questions, wish to get answers
here:
1.RNA or DNA, The tumor sample will be provided by a colleague in Hospital.
I can get both DNA and RNA purified. Should I sequence DNA or RNA after I
PCR them out?
2.If only 1 copy has mutation and the other copy is wt, can routine sequence
in core lab detect it? Or I have to use the so-called pyrosequence?
Thanks!
I need to sequence PI3K and PTEN in tumors for determine whether there is
mutation. But I do not know much about sequencing mutations since I only
work kinase for years. So I have a few basic questions, wish to get answers
here:
1.RNA or DNA, The tumor sample will be provided by a colleague in Hospital.
I can get both DNA and RNA purified. Should I sequence DNA or RNA after I
PCR them out?
2.If only 1 copy has mutation and the other copy is wt, can routine sequence
in core lab detect it? Or I have to use the so-called pyrosequence?
Thanks!
p*g
4 楼
你在google就可以google到很多。基本上这个比TS更不重要，胡乱谢谢就可以了
n*y
5 楼
歌有点周吉伦的味道，唱的太一般了。
k*s
6 楼
The fast way is to PCR the target region and sequence. If only one mutation
, there will be two peaks at the specific sit and it can seen from the
sequencing chromatograph.
answers
.
I
sequence
【在 H*****e 的大作中提到】
: Hi experts,
: I need to sequence PI3K and PTEN in tumors for determine whether there is
: mutation. But I do not know much about sequencing mutations since I only
: work kinase for years. So I have a few basic questions, wish to get answers
: here:
: 1.RNA or DNA, The tumor sample will be provided by a colleague in Hospital.
: I can get both DNA and RNA purified. Should I sequence DNA or RNA after I
: PCR them out?
: 2.If only 1 copy has mutation and the other copy is wt, can routine sequence
: in core lab detect it? Or I have to use the so-called pyrosequence?
, there will be two peaks at the specific sit and it can seen from the
sequencing chromatograph.
answers
.
I
sequence
【在 H*****e 的大作中提到】
: Hi experts,
: I need to sequence PI3K and PTEN in tumors for determine whether there is
: mutation. But I do not know much about sequencing mutations since I only
: work kinase for years. So I have a few basic questions, wish to get answers
: here:
: 1.RNA or DNA, The tumor sample will be provided by a colleague in Hospital.
: I can get both DNA and RNA purified. Should I sequence DNA or RNA after I
: PCR them out?
: 2.If only 1 copy has mutation and the other copy is wt, can routine sequence
: in core lab detect it? Or I have to use the so-called pyrosequence?
I*t
7 楼
我google了，但是没有找到什么template。也知道不重要，不过还是想能有个参考，靠
谱些
谱些
l*u
8 楼
挺好听的。孙楠的好像稍好些，刘欢差点意思。
【在 l*******r 的大作中提到】
: 本届晚会里最好听的一首了
【在 l*******r 的大作中提到】
: 本届晚会里最好听的一首了
O*e
9 楼
1. I would get both DNA and RNA, just in case RNA-Seq becomes an option in
the future.
2. Yes.
answers
.
I
sequence
【在 H*****e 的大作中提到】
: Hi experts,
: I need to sequence PI3K and PTEN in tumors for determine whether there is
: mutation. But I do not know much about sequencing mutations since I only
: work kinase for years. So I have a few basic questions, wish to get answers
: here:
: 1.RNA or DNA, The tumor sample will be provided by a colleague in Hospital.
: I can get both DNA and RNA purified. Should I sequence DNA or RNA after I
: PCR them out?
: 2.If only 1 copy has mutation and the other copy is wt, can routine sequence
: in core lab detect it? Or I have to use the so-called pyrosequence?
the future.
2. Yes.
answers
.
I
sequence
【在 H*****e 的大作中提到】
: Hi experts,
: I need to sequence PI3K and PTEN in tumors for determine whether there is
: mutation. But I do not know much about sequencing mutations since I only
: work kinase for years. So I have a few basic questions, wish to get answers
: here:
: 1.RNA or DNA, The tumor sample will be provided by a colleague in Hospital.
: I can get both DNA and RNA purified. Should I sequence DNA or RNA after I
: PCR them out?
: 2.If only 1 copy has mutation and the other copy is wt, can routine sequence
: in core lab detect it? Or I have to use the so-called pyrosequence?
l*i
10 楼
我也想知道，好多学校有类似要求。
o*y
11 楼
原来叫蜀绣 宣传四川的 郭敬明的词
l*1
12 楼
LZ you can try
NGS 基因测序仪IonProton
References:
a)
“个人基因组测序”时代来临 只需1000美元
2012年04月01日
来源：新华网
总部位于美国加州的生命科学厂商LifeTechnologies公司，29日宣布在中国推出新的台式基因测序仪IonProton。借助
该技术产品，只需1000美元即可在一天时间内完成个人全基因组测序。这意味着基因测序技术有望走进临床实践，“飞
入寻常百姓家
//tech.ifeng.com/discovery/detail_2012_04/01/13603299_0.shtml
b)
NGS SNP analysis Software:
for example:
V-Phaser & V-Profiler: variant calling a visualization tools to identify
biological mutations in diverse populations
//www.broadinstitute.org/scientific-community/science/projects/viral-
genomics/v-phaser
//www.broadinstitute.org/scientific-community/software?criteria=Next%
20generation%20tools
//www.broadinstitute.org/scientific-community/software?criteria=RNA-seq
more try
//www.broadinstitute.org/scientific-community/software
and
//www.broadinstitute.org/scientific-community/data
//www.broadinstitute.org/
all above links are from:
//www.shigen.nig.ac.jp/rice/oryzabase/tool/tool.jsp;jsessionid=
0D7767EEC6B68517E0FDE8DDAA20F6A9.4_6
【在 O******e 的大作中提到】
: 1. I would get both DNA and RNA, just in case RNA-Seq becomes an option in
: the future.
: 2. Yes.
:
: answers
: .
: I
: sequence
NGS 基因测序仪IonProton
References:
a)
“个人基因组测序”时代来临 只需1000美元
2012年04月01日
来源：新华网
总部位于美国加州的生命科学厂商LifeTechnologies公司，29日宣布在中国推出新的台式基因测序仪IonProton。借助
该技术产品，只需1000美元即可在一天时间内完成个人全基因组测序。这意味着基因测序技术有望走进临床实践，“飞
入寻常百姓家
//tech.ifeng.com/discovery/detail_2012_04/01/13603299_0.shtml
b)
NGS SNP analysis Software:
for example:
V-Phaser & V-Profiler: variant calling a visualization tools to identify
biological mutations in diverse populations
//www.broadinstitute.org/scientific-community/science/projects/viral-
genomics/v-phaser
//www.broadinstitute.org/scientific-community/software?criteria=Next%
20generation%20tools
//www.broadinstitute.org/scientific-community/software?criteria=RNA-seq
more try
//www.broadinstitute.org/scientific-community/software
and
//www.broadinstitute.org/scientific-community/data
//www.broadinstitute.org/
all above links are from:
//www.shigen.nig.ac.jp/rice/oryzabase/tool/tool.jsp;jsessionid=
0D7767EEC6B68517E0FDE8DDAA20F6A9.4_6
【在 O******e 的大作中提到】
: 1. I would get both DNA and RNA, just in case RNA-Seq becomes an option in
: the future.
: 2. Yes.
:
: answers
: .
: I
: sequence
h*e
13 楼
记得好像只有UCSD有这个要求
【在 I******t 的大作中提到】
: 哪位写过？能不能给个模板借鉴一下？叩谢了！
【在 I******t 的大作中提到】
: 哪位写过？能不能给个模板借鉴一下？叩谢了！
j*5
14 楼
卡拉OK的水平
【在 n***y 的大作中提到】
: 歌有点周吉伦的味道，唱的太一般了。
【在 n***y 的大作中提到】
: 歌有点周吉伦的味道，唱的太一般了。
a*k
15 楼
只有牛校才有这个要求。
我看到UT AUSTIN就要这个。结果。。。没敢申。
我看到UT AUSTIN就要这个。结果。。。没敢申。
l*r
16 楼
我说用“锦绣”怎么搜不到呢。原版的更好听啊。没看过郭敬明的书，从这个词看出他
还是有才
【在 o**y 的大作中提到】
: 原来叫蜀绣 宣传四川的 郭敬明的词
还是有才
【在 o**y 的大作中提到】
: 原来叫蜀绣 宣传四川的 郭敬明的词
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