Troubleshooting for SDS-PAGE. Smear on low molecular proteins - 未名空间MITBBS历史存档

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Troubleshooting for SDS-PAGE. Smear on low molecular proteins

Troubleshooting for SDS-PAGE. Smear on low molecular proteins# Biology - 生物学

x*u2012-04-21 07:04

1 楼

I have been running SDS-PAGE several times and cannot get clear band for low
molecular proteins. Any suggestions will be highly appreciated.
Attached is a gel picture I just took yesterday. (12% resolving gel and load
50 ug total E. coli cell lysate proteins).

p*p2012-04-21 07:04

2 楼

自己配的胶?买的?
可能是胶不好,也可能过热
可以降低电压,放冰盒里跑

x*o2012-04-21 07:04

3 楼

用tricine sds page好像对分子量小的蛋白效果比较好.

low
load

【在 x********u 的大作中提到】

: I have been running SDS-PAGE several times and cannot get clear band for low
: molecular proteins. Any suggestions will be highly appreciated.
: Attached is a gel picture I just took yesterday. (12% resolving gel and load
: 50 ug total E. coli cell lysate proteins).