After get extraction, why DNA quality is very bad? Can I still use it?
After get extraction, why DNA quality is very bad? Can I still use it?# Biology - 生物学
f*w
1 楼
还有别的便宜方法吗,谢谢
h*g
2 楼
After gel extraction, I checked the DNA using Nanodrop, the 260/280 is 1.5, 260/230 is 0.5. Can I still use the DNA? I will use the DNA as template to make RNA. Thanks.
m*D
3 楼
Elute by ddH2O? pH could affect the ratio. So use TE buffer to measure O.D. again.
h*g
4 楼
Yes, I eluted by ddH2O. So I should use TE buffer to elude? I will use it to make RNA. Can I still use the DNA to make RNA? Thanks.
.
【在 m*********D 的大作中提到】 : Elute by ddH2O? pH could affect the ratio. So use TE buffer to measure O.D. : again.
s*r
5 楼
probably not suitable for subsequent experiments. Is the DNA concentration normal? One possibility is ethanol carryover.
x*e
6 楼
是有这个现象。做连接没问题,转录就不确定了。 我一般不用nanodrop测,直接跑胶看回收质量
,
【在 h*********g 的大作中提到】 : After gel extraction, I checked the DNA using Nanodrop, the 260/280 is 1.5, : 260/230 is 0.5. Can I still use the DNA? I will use the DNA as template to : make RNA. Thanks.
m*D
7 楼
Yes,the DNA should be fine for transcription template. If you load on a gel, you may even see ssDNA bands. Better elude in TE. However if elude in ddH2O, you can add some NaCl solution to 100 mM.this helps to form dsDNA.