I used pcDNA3.1-gene for overexpression transfection. If I wanna perform flow cytometry analysis, shall I use transcient transfectants or stable clones after selection for 2 weeks??? Thank you very much...
y*d
4 楼
需要employer letter说明你回来继续为他们服务,雇用开始时间。
s*s
5 楼
case by case.
【在 k********u 的大作中提到】 : I used pcDNA3.1-gene for overexpression transfection. If I wanna perform : flow cytometry analysis, shall I use transcient transfectants or stable : clones after selection for 2 weeks??? Thank you very much...
b*s
6 楼
depends on what you are trying to see
【在 k********u 的大作中提到】 : I used pcDNA3.1-gene for overexpression transfection. If I wanna perform : flow cytometry analysis, shall I use transcient transfectants or stable : clones after selection for 2 weeks??? Thank you very much...
k*u
7 楼
i am trying to see the cell cycle phase distribution.......really don't know what i should use, transcient transfecion for 48hrs or stable clones for 2 weeks....
【在 b******s 的大作中提到】 : depends on what you are trying to see
【在 k********u 的大作中提到】 : i am trying to see the cell cycle phase distribution.......really don't know : what i should use, transcient transfecion for 48hrs or stable clones for 2 : weeks....
b*s
9 楼
R u trying to see the effect of transgene on cell cycle by transgene marker/ PI staining? Not an expert of cell cycle control,but why not go ahead and do your transfection, take some cells to check with flow at 48hr and do your colony selection side-by-side? you can compare the result 48hr vs 2 weeks later
know 2
【在 k********u 的大作中提到】 : i am trying to see the cell cycle phase distribution.......really don't know : what i should use, transcient transfecion for 48hrs or stable clones for 2 : weeks....