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Phage problem

Phage problem# Biology - 生物学

Z*42012-08-23 07:08

1 楼

已经有了一定的C++基础
但是最近研究面试题的时候发现code用java写简洁很多，可能是java的API比较全？
所以想学些java，哪些书比较好？
想自己去编写小工具什么的是不是要学习Windows API？如果是那么用什么书比较好？
谢谢~O(∩_∩)O~

l*82012-08-23 07:08

2 楼

Hi, are there anyone familiar with E coli?
I've screened some mutants and is trying to confirm them by doing site
mutagenesis and spot titration. But, during the process I found my
transformants which were streaked out twice, were contaminated by phage (
precipitates and clear o/n culture without many cells). When I am doing the
o/n culture, I found that if I add Kan, there would be a phage problem, but
if I didn't, there wouldn't. This makes me sooooooo confused and
disappointed. I wonder whether there are anybody who work with bacteria can
give me some suggestions. Thank you!
P.S.
I am now growing my cells in 37C with or without Kan at the same time to
figure out wheter it is antibiotics inducible.

Z*42012-08-23 07:08

3 楼

我找到了一个书叫《programming windows with MFC》MS press的怎么样？

b*y2012-08-23 07:08

4 楼

My guess,
Kan slow down the growth a bit. That allows phage to catch up and clear out
your culture.
Can you use TonA knockout strain to do your experiments? if so, you can try
that. (You can get the strain from a number of vendors.) The reason is that
the popular bacteria phage contamination is due to T1 phage. The receptor of
it on E. coli is TonA.
Good luck.

U*A2012-08-23 07:08

5 楼

MFC 不是很早就淘汰了？

l*82012-08-23 07:08

6 楼

Thank you so much!
My today's experiment also shows that after adding Kan, my bacteria got a
phage problem probably due to the reason you mentioned above.

out
try
that
of

【在 b******y 的大作中提到】

: My guess,
: Kan slow down the growth a bit. That allows phage to catch up and clear out
: your culture.
: Can you use TonA knockout strain to do your experiments? if so, you can try
: that. (You can get the strain from a number of vendors.) The reason is that
: the popular bacteria phage contamination is due to T1 phage. The receptor of
: it on E. coli is TonA.
: Good luck.