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谁来说说clonogenic survival assay

谁来说说clonogenic survival assay# Biology - 生物学

h*i2012-11-28 08:11

1 楼

我家现在厨房是electric stove.
water heater用的是 gas.
这块儿夏天一下雨或冬天一下雪就老停电，没法做饭。
于是想装一个备用的gas stove。一个灶眼就够了。
家里的natural gas的管道肯定是ready的，但不知从哪里下手。谁有这方面经验吗？先
谢过！

E*y2012-11-28 08:11

2 楼

这比一般什么XTT复杂很多，有什么优势？

j*72012-11-28 08:11

3 楼

找个local的做gas furnace的contractor来干就可以了，不贵。

s*l2012-11-28 08:11

4 楼

这玩意儿不是测量cell survival最直接的证据吗

【在 E**********y 的大作中提到】

: 这比一般什么XTT复杂很多，有什么优势？

n*e2012-11-28 08:11

5 楼

改煤气管道算大活了,heater 和 gas range 好像还不一样,还是请人搞吧.

【在 h***i 的大作中提到】

: 我家现在厨房是electric stove.
: water heater用的是 gas.
: 这块儿夏天一下雨或冬天一下雪就老停电，没法做饭。
: 于是想装一个备用的gas stove。一个灶眼就够了。
: 家里的natural gas的管道肯定是ready的，但不知从哪里下手。谁有这方面经验吗？先
: 谢过！

E*y2012-11-28 08:11

6 楼

XTT我觉得也很直接呀

x*h2012-11-28 08:11

7 楼

Anything related to gas needs permit from the local gov and gas company. Not
a DIY job!!!

【在 h***i 的大作中提到】

A*y2012-11-28 08:11

8 楼

The differences is not at the detection method. The cells grow differently
in cell culture (liquid) verses colongenic method (semi-solid). I can give
you a perfect example which is not reported anywhere. HDAC inhibitor, SAHA,
inhibits leukemia cell growth in cell culture (many papers). However, it
is useless against leukemia growth in colongenic assay.

【在 E**********y 的大作中提到】

: XTT我觉得也很直接呀

j*72012-11-28 08:11

9 楼

大什么大，一个小时都要不了。
就是再接跟管子而已。

【在 n*********e 的大作中提到】

: 改煤气管道算大活了,heater 和 gas range 好像还不一样,还是请人搞吧.

E*y2012-11-28 08:11

10 楼

is that why clonogenic survival assay becomes a standard assay to assess
therapeutic agents?

differently
give
SAHA,

【在 A******y 的大作中提到】

: The differences is not at the detection method. The cells grow differently
: in cell culture (liquid) verses colongenic method (semi-solid). I can give
: you a perfect example which is not reported anywhere. HDAC inhibitor, SAHA,
: inhibits leukemia cell growth in cell culture (many papers). However, it
: is useless against leukemia growth in colongenic assay.

h*i2012-11-28 08:11

11 楼

Thanks for all the kind replies.
Since I am going to use the gas stove mainly for a backup purpose (only need
it when there is a power outage), I am also thinking of just buying a
portable gas stove, which use a propane gas cylinder. Is this a good idea? I
appreciate any inputs.

A*y2012-11-28 08:11

12 楼

Yes, you need it before in-vivo.

【在 E**********y 的大作中提到】

: is that why clonogenic survival assay becomes a standard assay to assess
: therapeutic agents?
:
: differently
: give
: SAHA,