小白请教，用Myc tag 做IP好，还是用His tag pull down 好 - 未名空间MITBBS历史存档

小白请教，用Myc tag 做IP好，还是用His tag pull down 好# Biology - 生物学

i*r2012-12-27 08:12

1 楼

我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
一位置,这到底是怎么回事?
大家给分析一下.

G*d2012-12-27 08:12

2 楼

长疯了。都已经掐过一个顶了，侧枝也减过，侧芽也掐过。还长。是不管他还是继续掐
芽和封顶。花儿开了又谢。就是不长果子。

a*a2012-12-27 08:12

3 楼

【以下文字转载自 Sex 讨论区】
发信人: vt300 (vt300), 信区: Sex
标题: Re: 美国东北部堕胎求教？
发信站: BBS 未名空间站 (Thu Oct 14 03:01:28 2010, 美东)
女人让我睡一睡，我给你推荐一个好去处。

f*d2012-12-27 08:12

4 楼

我刚开始做蛋白质方面的东西，是小白，希望大家赐教！我有一个蛋白A，在C
terminal加了Myc tag 和his tag, 然后在hepG2里面超表达。我想用mass spec看在A蛋
白的interacting proteins, 这种情况是用Myc tag来做IP 好呢，还是直接把lysate上
Ni TNA 的柱子呢？

c*r2012-12-27 08:12

5 楼

看他们系里各自的支持者互掐的结果
如果没有人支持就洗洗睡了

m*62012-12-27 08:12

6 楼

几区的？是不是今年雨水比较多，热天也比较少？
我有棵也是这样，一颗就结了8个果。

c*n2012-12-27 08:12

7 楼

诺诺总是转这么色彩的

【在 a***a 的大作中提到】

: 【以下文字转载自 Sex 讨论区】
: 发信人: vt300 (vt300), 信区: Sex
: 标题: Re: 美国东北部堕胎求教？
: 发信站: BBS 未名空间站 (Thu Oct 14 03:01:28 2010, 美东)
: 女人让我睡一睡，我给你推荐一个好去处。

b*y2012-12-27 08:12

8 楼

Ni TNA 的柱子 is too nonspecific. But you can do Ni TNA 的柱子 and then Myc
tag来做IP.

c*p2012-12-27 08:12

9 楼

那印度哥们估计是选了去当面试炮灰的，你朋友要是去面试了他们中意的人选就悬了
[在 indydr () 的大作中提到：]
：
：
：...........

h*w2012-12-27 08:12

10 楼

天太热了？

a*a2012-12-27 08:12

11 楼

这是人间百态

【在 c*n 的大作中提到】

: 诺诺总是转这么色彩的

e*s2012-12-27 08:12

12 楼

单纯的蛋白蛋白interaction,我个人喜欢pulldown，因为下步Western不存在IGG干扰的
问题。不过Mass Spec就不知道了。建议是Imidazole特异洗脱，别用SDS buffer直接洗。
Myc做Co-IP还是不错的Tag，而且看见过几篇mass spec based proteomics用Myc.

【在 f*******d 的大作中提到】

: 我刚开始做蛋白质方面的东西，是小白，希望大家赐教！我有一个蛋白A，在C
: terminal加了Myc tag 和his tag, 然后在hepG2里面超表达。我想用mass spec看在A蛋
: 白的interacting proteins, 这种情况是用Myc tag来做IP 好呢，还是直接把lysate上
: Ni TNA 的柱子呢？

k*g2012-12-27 08:12

13 楼

怎么越在这个版混越感觉这里面的浑水，有点不寒而栗的感觉？

X*o2012-12-27 08:12

14 楼

身高暴露贴？嘿嘿

★ 发自iPhone App: ChineseWeb 7.8

【在 G******d 的大作中提到】

: 长疯了。都已经掐过一个顶了，侧枝也减过，侧芽也掐过。还长。是不管他还是继续掐
: 芽和封顶。花儿开了又谢。就是不长果子。

c*n2012-12-27 08:12

15 楼

赞阅历

【在 a***a 的大作中提到】

: 这是人间百态

f*d2012-12-27 08:12

16 楼

那用Imidazole洗脱之后，蛋白之间的interaction会被打断吗？洗脱之后还要exchange
buffer吗？还是直接可以加Myc Ab呢？

Myc

【在 b******y 的大作中提到】

: Ni TNA 的柱子 is too nonspecific. But you can do Ni TNA 的柱子 and then Myc
: tag来做IP.

M*P2012-12-27 08:12

17 楼

network

【在 i****r 的大作中提到】

: 我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
: 一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
: onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
: 一位置,这到底是怎么回事?
: 大家给分析一下.

c*e2012-12-27 08:12

18 楼

趁火打劫阿

【在 a***a 的大作中提到】

: 【以下文字转载自 Sex 讨论区】
: 发信人: vt300 (vt300), 信区: Sex
: 标题: Re: 美国东北部堕胎求教？
: 发信站: BBS 未名空间站 (Thu Oct 14 03:01:28 2010, 美东)
: 女人让我睡一睡，我给你推荐一个好去处。

f*d2012-12-27 08:12

19 楼

能不能给我一篇文章的link阿？我想看看方法和他们用哪家的antibody。谢啦～

洗。

【在 e****s 的大作中提到】

: 单纯的蛋白蛋白interaction,我个人喜欢pulldown，因为下步Western不存在IGG干扰的
: 问题。不过Mass Spec就不知道了。建议是Imidazole特异洗脱，别用SDS buffer直接洗。
: Myc做Co-IP还是不错的Tag，而且看见过几篇mass spec based proteomics用Myc.

s*02012-12-27 08:12

20 楼

我觉得拿到OFFER的关键有三个，一个是自己的基本条件要qualify, 第二个是自己的
方向和对方的要求非常吻合，第三个是有人挺你也就是network

a*a2012-12-27 08:12

21 楼

发信人: firingdragon (火云邪神的心，也有春天), 信区: Sex
标题: Re: 美国东北部堕胎求教？
发信站: BBS 未名空间站 (Thu Oct 14 03:28:50 2010, 美东)
要不我来妇科检查确认下，到底怀上没有
[1;33m【在 dzdzgxn (火眼) 的大作中提到: 】
[m
[36m: 各位前辈，小弟的一位朋友最近一不小心出了事故，现在大概怀孕两周左右，两
个人都 [m
[36m: 是学生，现在不知道怎么办好。
[m
[36m: 请问，在美国怀孕三个月以内可以做掉么？用什么样的方式？是否是在正规大医
院？费 [m
[36m: 用大概有多少？学生保险是否可以cover？
[m
[36m: 实在是非常感谢，我一倍儿好的哥们，如果需要包子或者转账的话，我会尽全力
的，多 [m
[36m: 谢多谢！！
[m
[36m
[m
[36m
[m
[36m
[m

【在 c********e 的大作中提到】

: 趁火打劫阿

e*s2012-12-27 08:12

22 楼

我懒啊。
自己搜吧，遍地都是。

【在 f*******d 的大作中提到】

: 能不能给我一篇文章的link阿？我想看看方法和他们用哪家的antibody。谢啦～
:
: 洗。

i*r2012-12-27 08:12

23 楼

楼上几位分析很有道理,但文章数比例大于30:1,最起码给人家一个电面机会.我认为,中
国人太艰难了,欧、美人不把中国人看在眼里也是一个重要因素.不知楼上几位认同这个
因素么？

B*e2012-12-27 08:12

24 楼

街上到处都是打胎的。。。

c*b2012-12-27 08:12

25 楼

应该不会，immidazole是histidine的mimic而已，而不是象高盐高detergent那样。

exchange

【在 f*******d 的大作中提到】

: 那用Imidazole洗脱之后，蛋白之间的interaction会被打断吗？洗脱之后还要exchange
: buffer吗？还是直接可以加Myc Ab呢？
:
: Myc

e*g2012-12-27 08:12

26 楼

我觉得你的信息可能有误，虽然眼睛瞎的人有，
一篇档次不高，且非第一作者的人，要进onsite的可能性太小。
除非是teaching 的位置。

【在 i****r 的大作中提到】

: 我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
: 一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
: onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
: 一位置,这到底是怎么回事?
: 大家给分析一下.

b*y2012-12-27 08:12

27 楼

Echo what centuribob said.

i*r2012-12-27 08:12

28 楼

我只是从Scienfinder搜索的,没有搜其他数据库,这个印度薄后是UIUC的,可能有点出入
,但不会偏得很远.

S*s2012-12-27 08:12

29 楼

// non-denature Tandem purification
3XFLAG + HA
or
ProteinA beads(IgG domain)+HA/FLAG
His or myc affinity and specific 不够~
//denature purification
His and biotag 会比较好~

【在 f*******d 的大作中提到】

: 我刚开始做蛋白质方面的东西，是小白，希望大家赐教！我有一个蛋白A，在C
: terminal加了Myc tag 和his tag, 然后在hepG2里面超表达。我想用mass spec看在A蛋
: 白的interacting proteins, 这种情况是用Myc tag来做IP 好呢，还是直接把lysate上
: Ni TNA 的柱子呢？

h*02012-12-27 08:12

30 楼

我倾向你的判断。招人是一个大的系统工程，没那么容易随便onsite。
一个committee 里面好几个人，你凭什么就让只有一篇文章的人来。不可能。

【在 e****g 的大作中提到】

: 我觉得你的信息可能有误，虽然眼睛瞎的人有，
: 一篇档次不高，且非第一作者的人，要进onsite的可能性太小。
: 除非是teaching 的位置。

f*d2012-12-27 08:12

31 楼

多谢！ proteinA beads (IgG domain) 这个是啥意思？你说的连个都是tandem 的吗？

【在 S*********s 的大作中提到】

: // non-denature Tandem purification
: 3XFLAG + HA
: or
: ProteinA beads(IgG domain)+HA/FLAG
: His or myc affinity and specific 不够~
: //denature purification
: His and biotag 会比较好~

T*g2012-12-27 08:12

32 楼

我觉得除非有什么证据，不要轻易把原因归在种族上。
其他可能的因素太多了。

【在 i****r 的大作中提到】

: 楼上几位分析很有道理,但文章数比例大于30:1,最起码给人家一个电面机会.我认为,中
: 国人太艰难了,欧、美人不把中国人看在眼里也是一个重要因素.不知楼上几位认同这个
: 因素么？

U*m2012-12-27 08:12

33 楼

哈哈哈

【在 e****s 的大作中提到】

: 我懒啊。
: 自己搜吧，遍地都是。

w*h2012-12-27 08:12

34 楼

赞“我的一位朋友”。。。
别人的CV详细如何，如果没有放在网上，你未必清楚。。。

【在 i****r 的大作中提到】

: 我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
: 一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
: onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
: 一位置,这到底是怎么回事?
: 大家给分析一下.

f*d2012-12-27 08:12

35 楼

又有一个问题，还是贴在这里把，要做IP看interacting protein, lysate是不是一定
不能用sonication, 只能用detergent来lyse cell?

h*w2012-12-27 08:12

36 楼

文章只是一个方面，我们以前也面过只有非常少文章的一个人（少于5篇，而且没有第
一作者），但是人家刚拿到50万的GRANT，这个比30篇文章都管用

a*e2012-12-27 08:12

37 楼

也知道类似的情况，一片文章都没有的人照样拿几十万的房顶，然后人学校直接给位置
；一篇文章都没有的，没毕业直接拿到TT ap。当然这些都是大牛们力挺的，嘿嘿
如果自己没啥好资本跟别人较量，最好放平心态。能拿到更好，没有也无所谓。30年河
东，30年河西，现在也许你没还拿到工作，多年后，没准你比比尔盖茨还富有/辉煌，
哈哈哈

【在 h*****w 的大作中提到】

: 文章只是一个方面，我们以前也面过只有非常少文章的一个人（少于5篇，而且没有第
: 一作者），但是人家刚拿到50万的GRANT，这个比30篇文章都管用

A*a2012-12-27 08:12

38 楼

When we review the applicant’s CV, we consider the following aspects:
1。你的毕业学校好于申请学校。至少同一档次。Otherwise, unless you are an
experienced AP with good research and funding records, you have little
chance to be considered. Are you from a leading research group? Is your
advisor famous?
2。文章quantity and quality 满足基本要求。very important. You need to have
at least 3~5 first authored papers published in top journals in your area
for a fresh phd。If you have GOOGLE CITATION REPORT showing that most of
your papers are well referenced, that is very helpful. For folks from
industry, we need to see if you have a gap in your publication record.
3. Are you in the right area? Very important. You won’t stand a chance if
your area overlaps with the current faculty’s research areas and they do
not want to hire people in the area. Normally each faculty in the search
committee represents an area and they turns to support their candidates with
greater energy. So make sure you read between lines and get some insider
information about exactly which area is hiring and write your research
statement to fit yourself in.
4. Funding application experience. Very important. If you are PIs of big
NSF, industry, or government funding, you will have a very good chance to
get a phone interview unless your area of interests overlap with another
professor in the same department and they don’t want to hire anyone in that
area any more.
5. Do you have teaching experience (I don't think research oriented schools
take it seriously enough) are you teaching courses we need people to teach?
Do you have high class evaluation? Is this course going to be a popular
course? Not that important aspect – not going to be so important that
people will kill your package because you do not show good teaching
potentials.
6. Do you switch jobs very often?
7. service. Are you editors of top journals? Etc.
From research statement
Is it well written? Is this research statement good enough to attract
funding? Are you an independent researcher? Do you have your own research
projects? Is your research area a promising one? Do you need big money to
start? Etc.

【在 i****r 的大作中提到】

: 我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
: 一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
: onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
: 一位置,这到底是怎么回事?
: 大家给分析一下.

d*h2012-12-27 08:12

39 楼

UF去年几个印度人就基本上一手遮天了
那onsite的都是啥烂货啊
还差点把某个committee member的弟弟招了
第一次见这么不要脸的

【在 h********0 的大作中提到】

: 我倾向你的判断。招人是一个大的系统工程，没那么容易随便onsite。
: 一个committee 里面好几个人，你凭什么就让只有一篇文章的人来。不可能。

i*r2012-12-27 08:12

40 楼

楼上有些同胞的分析很犀利.令人感觉不爽.
我只是为我那位朋友感到有点不解,他自己无所谓,因为他有过几个工业界面试,OFFER离
他很近了.
不过Adama的信息很实在,让人觉得很舒服.不过想诚心请教Adama一个问题:Do you
switch jobs very often? 如果真地换过几个地方,那怎么办?在简历中去掉几段经历?
能行吗?
谢谢!

c*p2012-12-27 08:12

41 楼

自己就是自己，一个朋友一个朋友的有意思吗？
[在 indydr () 的大作中提到：]
：
：
：...........

i*r2012-12-27 08:12

42 楼

crabpp, 你到底想干什么?你的智商超过10000了? 千里眼看见是一个人?
有建议就提,不要搅混水.
我朋友与我同一系,不同方向,你说说,我为何要把自己说成别人,有病呀!

m*m2012-12-27 08:12

43 楼

为了一个薪水不高的工作要把自己锻炼成无所不能的铁人，满足所有的条件，太难了
。

k*g2012-12-27 08:12

44 楼

问题是申请的学校大多不知道谁是scc里，so we do not have any idea how well our
research
fits or if there is any conflict.

【在 A***a 的大作中提到】

: When we review the applicant’s CV, we consider the following aspects:
: 1。你的毕业学校好于申请学校。至少同一档次。Otherwise, unless you are an
: experienced AP with good research and funding records, you have little
: chance to be considered. Are you from a leading research group? Is your
: advisor famous?
: 2。文章quantity and quality 满足基本要求。very important. You need to have
: at least 3~5 first authored papers published in top journals in your area
: for a fresh phd。If you have GOOGLE CITATION REPORT showing that most of
: your papers are well referenced, that is very helpful. For folks from
: industry, we need to see if you have a gap in your publication record.

x*52012-12-27 08:12

45 楼

这种情况下只能广撒网，同时充分发挥networking

research

【在 k******g 的大作中提到】

: 问题是申请的学校大多不知道谁是scc里，so we do not have any idea how well our
: research
: fits or if there is any conflict.

k*g2012-12-27 08:12

46 楼

谢谢了。不过我的同学以及学姐学兄的，80%毕业后直接就进入了大公司了。
只有我比较喜欢学术界，一心在这苦琢磨，呵呵；）

【在 x*******5 的大作中提到】

: 这种情况下只能广撒网，同时充分发挥networking
:
: research

A*a2012-12-27 08:12

47 楼

A few suggestions for those of you who are still preparing your package and
thinking of applying the faculty position next year.
1) Before you apply, make sure you already know you fit for the school you
apply for. If you don't do your homework and you are not a superstar, no
matter how many schools you apply for, you will have very little chance to
get even a phone interview.
-- with your publiation record, the ranking of the school you graduated,
the reputation of your advisor, the funding record, you can already have a
pretty good idea which school will consider your application. I can tell
you among hundreds of applications we received, only those fit the
preliminary requirements will be reviewed carefully. The time each faculty
spends on screening a resume may be less than 1 minute. After you pass the
screening, they start to read your research statement. Then your teaching
statement.

**** a few examples of screening rule of thumb for a relatively good scholl
If you are a fresh phd from a lower-ranking school without a stanning record
(super good papers; a great well-networked professor; awards); your
application package may not even be read.
If you are a fresh phd from an ok school with very few good publications and
an area no listed on the interest list; you are not competitive
Exceptions of the above examples:
If you are a post-doc now at a good school with 2~3 years experience with
good publication record and you start to write proposals, poor ranking of
your phd school can be partially ignored.
If you are recommended by some faculty in the department, your school
ranking can partially be ignored.
If you are working in an area that is very hot and it is not possible to get
a candidate from a better school, you can be considered.
**************
2) Research direction can be found out from the faculty webpages, so please
do your homework to see whether or not you have an overlap and if you can
bring something new to the department. You can also through the
publications of the professors understand what they do in the area. You can
ask the students in the school to understand what is going on in an
department. Belive me, if you are not prepared till this end, you will fail
your phone interview any way. I don't think those people who apply for a
good school should apply many to increase your chance. I think those who,
before they apply, already know that they stand a chance will be the ones
entering the short list. There are just too many applications (at least 40%
of the packages I reviewed) be screened out in 30 seconds.
3) It may be unfair but everything drills down to
- graduate from a good school or advisor is famous
- excellent publication record
- funding
- match research needs and future direction of the department
4) regarding job switching. If you switch jobs ever 2 years, even for
postdoc positions, it means you don't have a good continuity in the work you
do and you seem to lack of a focus. The department also wants to know when
we have you here, whether or not we are your jummp board to other schools.
Depending on the nature, some search committee members may consider it is a
negative sign. Not that critical.
5) If you got a phone interview
- Communication skill. If your English is poor and you cann't communicate
your research ideas effectively, you stand zero chance. As nowadays, we have
more qualified candidates than needed. If you go through the old posts,
you will find plenty of suggestions about how to address those interview
questions so be prepared is not a hard task.
- Understand the department. If you do little homework on what is going on
in the school you applied for, now is the time to pay. It is very easy to
figure out whether or not an applicant did their homework by asking them how
their research can fit in our program and to whom they want to collaborate,
etc..
- often times, we will clarify some doubts we have on your application
package.
When serving in the search committee, I think our goal is to make the
department stronger and find ourselves a good person to work with. For
those schools who can not form a search committee that is fair to all
applicants, you will have a hard time in the future to survive there. It is
to your best interest you are not on their list.
Hope this post will be helpful for you to prepare your application package.
Good luck for those who truly want to start a career in academia.

our

【在 k******g 的大作中提到】

: 问题是申请的学校大多不知道谁是scc里，so we do not have any idea how well our
: research
: fits or if there is any conflict.

i*r2012-12-27 08:12

48 楼

谢谢Adama祥细又很到位的分析,这样的贴子真正让人受益,越多越好!

n*l2012-12-27 08:12

49 楼

这个很精准。

and

【在 A***a 的大作中提到】

: A few suggestions for those of you who are still preparing your package and
: thinking of applying the faculty position next year.
: 1) Before you apply, make sure you already know you fit for the school you
: apply for. If you don't do your homework and you are not a superstar, no
: matter how many schools you apply for, you will have very little chance to
: get even a phone interview.
: -- with your publiation record, the ranking of the school you graduated,
: the reputation of your advisor, the funding record, you can already have a
: pretty good idea which school will consider your application. I can tell
: you among hundreds of applications we received, only those fit the

X*22012-12-27 08:12

50 楼

可能文章太多，系里的教授们容不下他。

【在 i****r 的大作中提到】

: 我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
: 一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
: onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
: 一位置,这到底是怎么回事?
: 大家给分析一下.

a*k2012-12-27 08:12

51 楼

Adama肯定在一个good research school而且是一个积极向上的系。
有些学校已经在走下坡路的，招人就随意的多。很烂的人都会招的。

and

【在 A***a 的大作中提到】

: A few suggestions for those of you who are still preparing your package and
: thinking of applying the faculty position next year.
: 1) Before you apply, make sure you already know you fit for the school you
: apply for. If you don't do your homework and you are not a superstar, no
: matter how many schools you apply for, you will have very little chance to
: get even a phone interview.
: -- with your publiation record, the ranking of the school you graduated,
: the reputation of your advisor, the funding record, you can already have a
: pretty good idea which school will consider your application. I can tell
: you among hundreds of applications we received, only those fit the

m*m2012-12-27 08:12

52 楼

这种专业一般挺好找到教职的，大部分phd都去工业界了，学界竞争压力没那么大。

【在 k******g 的大作中提到】

: 谢谢了。不过我的同学以及学姐学兄的，80%毕业后直接就进入了大公司了。
: 只有我比较喜欢学术界，一心在这苦琢磨，呵呵；）

I*c2012-12-27 08:12

53 楼

skin color is the key

k*g2012-12-27 08:12

54 楼

thanks a lot.
收藏了。

and

【在 A***a 的大作中提到】

: A few suggestions for those of you who are still preparing your package and
: thinking of applying the faculty position next year.
: 1) Before you apply, make sure you already know you fit for the school you
: apply for. If you don't do your homework and you are not a superstar, no
: matter how many schools you apply for, you will have very little chance to
: get even a phone interview.
: -- with your publiation record, the ranking of the school you graduated,
: the reputation of your advisor, the funding record, you can already have a
: pretty good idea which school will consider your application. I can tell
: you among hundreds of applications we received, only those fit the

c*e2012-12-27 08:12

55 楼

多谢！看您的帖子，每次都受益匪浅！今年我从工业界申请faculty，在工业界做了3年
，大部分文章都是合作，不是一作，倒是有一些会议报告。这样的算有gap么？还有
funding的问题，因为在研发部门，funding都是公司的，内部申请还是比NSF, DOE 之
类的容易很多，这样的话要怎么在申请中介绍这种funding呢，毕竟不是一个level的，
虽然钱不少。

【在 A***a 的大作中提到】

: When we review the applicant’s CV, we consider the following aspects:
: 1。你的毕业学校好于申请学校。至少同一档次。Otherwise, unless you are an
: experienced AP with good research and funding records, you have little
: chance to be considered. Are you from a leading research group? Is your
: advisor famous?
: 2。文章quantity and quality 满足基本要求。very important. You need to have
: at least 3~5 first authored papers published in top journals in your area
: for a fresh phd。If you have GOOGLE CITATION REPORT showing that most of
: your papers are well referenced, that is very helpful. For folks from
: industry, we need to see if you have a gap in your publication record.

s*g2012-12-27 08:12

56 楼

工业界申请faculty一般很难很难吧，除非做到很高级别的

【在 c*****e 的大作中提到】

: 多谢！看您的帖子，每次都受益匪浅！今年我从工业界申请faculty，在工业界做了3年
: ，大部分文章都是合作，不是一作，倒是有一些会议报告。这样的算有gap么？还有
: funding的问题，因为在研发部门，funding都是公司的，内部申请还是比NSF, DOE 之
: 类的容易很多，这样的话要怎么在申请中介绍这种funding呢，毕竟不是一个level的，
: 虽然钱不少。

v*y2012-12-27 08:12

57 楼

Thanks so much.

and

【在 A***a 的大作中提到】

: A few suggestions for those of you who are still preparing your package and
: thinking of applying the faculty position next year.
: 1) Before you apply, make sure you already know you fit for the school you
: apply for. If you don't do your homework and you are not a superstar, no
: matter how many schools you apply for, you will have very little chance to
: get even a phone interview.
: -- with your publiation record, the ranking of the school you graduated,
: the reputation of your advisor, the funding record, you can already have a
: pretty good idea which school will consider your application. I can tell
: you among hundreds of applications we received, only those fit the

p*y2012-12-27 08:12

58 楼

Well said.
Industry experience is a plus.
Fit to the area is sometimes more important.

T*92012-12-27 08:12

59 楼

也许是系里某两个 "I" 打头的国家faculty 多吧
淘汰中国candidate用得最多的理由是 "communication issue", 虽然电话那头
interview 你的人不知道说的是不是英语呢，而你表达的很清楚。
没办法，中国年轻人除了必须实力强外，往往还要承受一些额外的一些东西...
想当faculty 的，没事抓住各种机会多练英语，如果到了想挺你的人都觉得和你交流有
问题的话，就难了。

【在 i****r 的大作中提到】

: 我还有点真迷糊:拿到onsite或offer的条件到底是什么? 我观察到:有一个印度薄后才
: 一篇档次不高的文章(Scinfinder 搜索,非第一作者)拿到一个排名一百左右学校的
: onsite,我的一个朋友(中国同胞)有30多篇文章却没有电面机会,他们是申请同一学校同
: 一位置,这到底是怎么回事?
: 大家给分析一下.