f*7
2 楼
用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
conditioned media of cell cuture).
it looks stranger.
this is possible?
or the design of primer of pcr is not right?
thanks
conditioned media of cell cuture).
it looks stranger.
this is possible?
or the design of primer of pcr is not right?
thanks
s*s
3 楼
啥protein array, western做了没？
【在 f*********7 的大作中提到】
: 用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
: conditioned media of cell cuture).
: it looks stranger.
: this is possible?
: or the design of primer of pcr is not right?
: thanks
【在 f*********7 的大作中提到】
: 用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
: conditioned media of cell cuture).
: it looks stranger.
: this is possible?
: or the design of primer of pcr is not right?
: thanks
D*a
4 楼
不答就罚站...
l*1
5 楼
北美魁镇的位置的interview 机会 拿到没?
if still not yet,
please try
Bourses postdoctorales 2013-2014
DERNIER DéLAI : 1er mars 2013
HTTP: //www.cerium.ca/Bourses-postdoctorales-2012-2013
----------
or alternatively
Bourse postdoctorale (B3)
ATTENTION - IMPORTANT
Il existe présentement deux versions du CV commun canadien, mais UNE SEULE
d'entre elles peut être soumise aux Fonds de recherche du Québec. Elle est
disponible à la nouvelle adresse suivante : www.ccv-cvc-2004.ca
web link:
HTTP: //www.fqrsc.gouv.qc.ca/fr/bourses/programme.php?id_programme=20
-----
HTTP: //translate.google.fr/?hl=fr
Postdoctoral Fellowship (B3)
ATTENTION - IMPORTANT
There are currently two versions of the Canadian Common CV, but ONE of them
may be subject to the Research Fund of Quebec. It is available at the
following new address: cvc-www.ccv-2004.ca
ps: 只针对枫叶国绿卡或北美护照的 PhD/MD 哈
【在 D*a 的大作中提到】
: 不答就罚站...
if still not yet,
please try
Bourses postdoctorales 2013-2014
DERNIER DéLAI : 1er mars 2013
HTTP: //www.cerium.ca/Bourses-postdoctorales-2012-2013
----------
or alternatively
Bourse postdoctorale (B3)
ATTENTION - IMPORTANT
Il existe présentement deux versions du CV commun canadien, mais UNE SEULE
d'entre elles peut être soumise aux Fonds de recherche du Québec. Elle est
disponible à la nouvelle adresse suivante : www.ccv-cvc-2004.ca
web link:
HTTP: //www.fqrsc.gouv.qc.ca/fr/bourses/programme.php?id_programme=20
-----
HTTP: //translate.google.fr/?hl=fr
Postdoctoral Fellowship (B3)
ATTENTION - IMPORTANT
There are currently two versions of the Canadian Common CV, but ONE of them
may be subject to the Research Fund of Quebec. It is available at the
following new address: cvc-www.ccv-2004.ca
ps: 只针对枫叶国绿卡或北美护照的 PhD/MD 哈
【在 D*a 的大作中提到】
: 不答就罚站...
D*a
6 楼
多谢！ 没呢...
忙着乱七八糟的事...
SEULE
est
【在 l**********1 的大作中提到】
: 北美魁镇的位置的interview 机会 拿到没?
: if still not yet,
: please try
: Bourses postdoctorales 2013-2014
: DERNIER DéLAI : 1er mars 2013
: HTTP: //www.cerium.ca/Bourses-postdoctorales-2012-2013
: ----------
: or alternatively
: Bourse postdoctorale (B3)
: ATTENTION - IMPORTANT
忙着乱七八糟的事...
SEULE
est
【在 l**********1 的大作中提到】
: 北美魁镇的位置的interview 机会 拿到没?
: if still not yet,
: please try
: Bourses postdoctorales 2013-2014
: DERNIER DéLAI : 1er mars 2013
: HTTP: //www.cerium.ca/Bourses-postdoctorales-2012-2013
: ----------
: or alternatively
: Bourse postdoctorale (B3)
: ATTENTION - IMPORTANT
l*1
7 楼
De rien.
NB:
deadline: March 1st 2013 for above Québec PD fellowship.
NB:
deadline: March 1st 2013 for above Québec PD fellowship.
p*p
8 楼
A positive control for realtime PCR is needed.
【在 f*********7 的大作中提到】
: 用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
: conditioned media of cell cuture).
: it looks stranger.
: this is possible?
: or the design of primer of pcr is not right?
: thanks
【在 f*********7 的大作中提到】
: 用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
: conditioned media of cell cuture).
: it looks stranger.
: this is possible?
: or the design of primer of pcr is not right?
: thanks
b*n
9 楼
引物设计有问题，可能落在intron里面了
或者是alternative splicing，你们检测的那种isoform不占大头
【在 f*********7 的大作中提到】
: 用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
: conditioned media of cell cuture).
: it looks stranger.
: this is possible?
: or the design of primer of pcr is not right?
: thanks
或者是alternative splicing，你们检测的那种isoform不占大头
【在 f*********7 的大作中提到】
: 用rt-pcr監測不到gene表答 (in cell), 但protein array監測蛋白表答 (in
: conditioned media of cell cuture).
: it looks stranger.
: this is possible?
: or the design of primer of pcr is not right?
: thanks
y*s
10 楼
one is false positive/negative...
f*7
11 楼
it is Qualitative Protein Microarray. the basic mechanism is similar to
western blotting.
sorry for the confusion
thanks
【在 s******s 的大作中提到】
: 啥protein array, western做了没？
western blotting.
sorry for the confusion
thanks
【在 s******s 的大作中提到】
: 啥protein array, western做了没？
s*s
12 楼
你是说quantitative? 不知道你这个怎么work的？感觉protein array
应该相当于elisa，没法解决non-specific的问题，和WB不能比
【在 f*********7 的大作中提到】
: it is Qualitative Protein Microarray. the basic mechanism is similar to
: western blotting.
: sorry for the confusion
: thanks
应该相当于elisa，没法解决non-specific的问题，和WB不能比
【在 f*********7 的大作中提到】
: it is Qualitative Protein Microarray. the basic mechanism is similar to
: western blotting.
: sorry for the confusion
: thanks
f*7
13 楼
this protein microarray has positive and negative control. both is normal (
positive control is postive and negative control is negative). do you think
there is still non-specifice problem?
I know primer design may be one problem. but how we excluded the non-
specific result?
thank you so much
【在 s******s 的大作中提到】
: 你是说quantitative? 不知道你这个怎么work的？感觉protein array
: 应该相当于elisa，没法解决non-specific的问题，和WB不能比
positive control is postive and negative control is negative). do you think
there is still non-specifice problem?
I know primer design may be one problem. but how we excluded the non-
specific result?
thank you so much
【在 s******s 的大作中提到】
: 你是说quantitative? 不知道你这个怎么work的？感觉protein array
: 应该相当于elisa，没法解决non-specific的问题，和WB不能比
L*S
14 楼
确定蛋白不是FBS里面的？
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