1. Do you see the B cell percentage increase in spleen,blood, and LNs? 2. Do you see the B cell subset changes in the lymphoid organs? MZ B cells, FO B cells, GC B cells. In the BM, there are quite many immature B cells. Which subset of B cells preferentially increases in the BM? 3. Do you see the level of changes in IgG, IgM, IgA in serum? 4. Is the effect B cell intrinsic? Say, to have mixed chimera, do you see selective KO B cell accumulation. No one can give you any answer based on your limited data information. If you want to pursue it further, you have to conduct the experiment listed 1-4.
谢谢。 1. the percentage of B cell in PB is also increased, not check SPL yet. 2. mainly, immature B cell increased in my KO mice compared to age-matched WT mice. As for MZ B cell, FO B cell, and GC B cell, I did not checked it yet. I plan to check pro-B, pre-B, pre-pro-B. 3. As for IgG, IgM, IgA, I did not check it yet. I can check it by ELISA. 4. I did not get your point. Some of my experimental result (mutual transplantation assay) shows that B cell increase in response to this gene deletion is intrinsically regulated. 5. One more data is that I found that Cd4+ cell in BM is also increased in this KO mice compared to age-matched WT mice.
cells, . -4.
【在 s****9 的大作中提到】 : 1. Do you see the B cell percentage increase in spleen,blood, and LNs? : 2. Do you see the B cell subset changes in the lymphoid organs? MZ B cells, : FO B cells, GC B cells. In the BM, there are quite many immature B cells. : Which subset of B cells preferentially increases in the BM? : 3. Do you see the level of changes in IgG, IgM, IgA in serum? : 4. Is the effect B cell intrinsic? Say, to have mixed chimera, do you see : selective KO B cell accumulation. : No one can give you any answer based on your limited data information. If : you want to pursue it further, you have to conduct the experiment listed 1-4. :
IgG, IgM, IgA, IgE level in serum? The antibody titter to vaccine? For T cell, measure the culture's th1, th2, T reg cell 数量ratio? and IFN-r, IL4, IL10的含量?
【在 w******y 的大作中提到】 : IgG, IgM, IgA, IgE level in serum? : The antibody titter to vaccine? : For T cell, measure the culture's th1, th2, T reg cell 数量ratio? and IFN-r, : IL4, IL10的含量?