qPCR 计算问题# Biology - 生物学
e*p
1 楼
记得有人问过但找不到帖子了,
三次重复试验,每次QPCR有三个TECH REP
Rep1
GOI-Houskeep
delta Ct: Con -2.9; Treatment: 2.1
Rep2
GOI-Houskeep
delta Ct: Con -1.9; Treatment: 2.0
Rep3
GOI-Houskeep
detla Ct: Con -2.1; Treatment: 3.0
请问如何综合三次结果用DELTA DELTA CT的方法算出 fold change +- 标准误啊,而且
把CON的平均值算成1
请高人指点
三次重复试验,每次QPCR有三个TECH REP
Rep1
GOI-Houskeep
delta Ct: Con -2.9; Treatment: 2.1
Rep2
GOI-Houskeep
delta Ct: Con -1.9; Treatment: 2.0
Rep3
GOI-Houskeep
detla Ct: Con -2.1; Treatment: 3.0
请问如何综合三次结果用DELTA DELTA CT的方法算出 fold change +- 标准误啊,而且
把CON的平均值算成1
请高人指点