important tip in cell selection Primary cells should be used. We have found that it is difficult to reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro , while fresh MEF are competent. Cells from mice older than one week showed very poor reprogramming efficiency under the current protocol. hmmm......
【在 z*t 的大作中提到】 : important tip in cell selection : Primary cells should be used. We have found that it is difficult to : reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro : , while fresh MEF are competent. : Cells from mice older than one week showed very poor reprogramming : efficiency under the current protocol. : hmmm......
d*e
43 楼
恭喜恭喜
D*a
44 楼
我们用e12的皮,酸泡完之后每天死一批,最后死的基本上都不剩神马了... 可能需要用很多很多细胞
vitro
【在 z*t 的大作中提到】 : important tip in cell selection : Primary cells should be used. We have found that it is difficult to : reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro : , while fresh MEF are competent. : Cells from mice older than one week showed very poor reprogramming : efficiency under the current protocol. : hmmm......
P*n
45 楼
pai
l*y
46 楼
今天有人post了一个新的comment,如果是真的大家就散了吧 "I read very interesting analysis by bioinformatics professional in RIKEN. Analyzing the input of ChIP-seq data published, both STAP and STAP stem cell s had no TCR-alpha and beta rearrangements. More surprisingly, original T cells for STAP cell production were female, bu t STAP and STAP stem cells were male."
【在 l***y 的大作中提到】 : 今天有人post了一个新的comment,如果是真的大家就散了吧 : "I read very interesting analysis by bioinformatics professional in RIKEN. : Analyzing the input of ChIP-seq data published, both STAP and STAP stem cell : s had no TCR-alpha and beta rearrangements. : More surprisingly, original T cells for STAP cell production were female, bu : t STAP and STAP stem cells were male."
important tip in cell selection Primary cells should be used. We have found that it is difficult to reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro , while fresh MEF are competent. Cells from mice older than one week showed very poor reprogramming efficiency under the current protocol. hmmm......
【在 z*t 的大作中提到】 : important tip in cell selection : Primary cells should be used. We have found that it is difficult to : reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro : , while fresh MEF are competent. : Cells from mice older than one week showed very poor reprogramming : efficiency under the current protocol. : hmmm......
D*a
94 楼
我们用e12的皮,酸泡完之后每天死一批,最后死的基本上都不剩神马了... 可能需要用很多很多细胞
vitro
【在 z*t 的大作中提到】 : important tip in cell selection : Primary cells should be used. We have found that it is difficult to : reprogram mouse embryonic fibroblasts (MEF) that have been expanded in vitro : , while fresh MEF are competent. : Cells from mice older than one week showed very poor reprogramming : efficiency under the current protocol. : hmmm......
l*y
95 楼
今天有人post了一个新的comment,如果是真的大家就散了吧 "I read very interesting analysis by bioinformatics professional in RIKEN. Analyzing the input of ChIP-seq data published, both STAP and STAP stem cell s had no TCR-alpha and beta rearrangements. More surprisingly, original T cells for STAP cell production were female, bu t STAP and STAP stem cells were male."
【在 l***y 的大作中提到】 : 今天有人post了一个新的comment,如果是真的大家就散了吧 : "I read very interesting analysis by bioinformatics professional in RIKEN. : Analyzing the input of ChIP-seq data published, both STAP and STAP stem cell : s had no TCR-alpha and beta rearrangements. : More surprisingly, original T cells for STAP cell production were female, bu : t STAP and STAP stem cells were male."
【在 l***y 的大作中提到】 : 今天有人post了一个新的comment,如果是真的大家就散了吧 : "I read very interesting analysis by bioinformatics professional in RIKEN. : Analyzing the input of ChIP-seq data published, both STAP and STAP stem cell : s had no TCR-alpha and beta rearrangements. : More surprisingly, original T cells for STAP cell production were female, bu : t STAP and STAP stem cells were male."