questions about mMESSENGERmMACHINE sp6# Biology - 生物学
j*3
1 楼
biotech方向 哪个学院更好？除了soft/hard money的区别以外 其他有没有特别的不同
？medical school是不是funding压力会很大?
？medical school是不是funding压力会很大?
w*r
2 楼
童鞋们，
首先祝愿大家绿卡申请顺利。
我打算自己提交ＥＢ－１Ｂ，ｐｅｔｉｔｉｏｎ letter 是以单位领导的口气写的。现
在想 efile and PP. 有些问题不是很清楚，请大家有经验的或者知道如何解决的帮助
一下。
１，在选择是自己为自己申请还是选择attorney 申请呢？
2,请问１４０还需不需要单位签字
３，请问其中的A#应该是在哪里找到啊？
多谢你们！
首先祝愿大家绿卡申请顺利。
我打算自己提交ＥＢ－１Ｂ，ｐｅｔｉｔｉｏｎ letter 是以单位领导的口气写的。现
在想 efile and PP. 有些问题不是很清楚，请大家有经验的或者知道如何解决的帮助
一下。
１，在选择是自己为自己申请还是选择attorney 申请呢？
2,请问１４０还需不需要单位签字
３，请问其中的A#应该是在哪里找到啊？
多谢你们！
h*g
3 楼
I am using Ambion mMESSENGERmMACHINE Sp6 to make mRNA from plasmid. After
making mRNA, I run a simple agarose gel. There are two bands. One band is
not very close to the actual size (400bp smaller), there is also another
smaller and stronger band. Can I still use the mRNA? Why there are two bands
? Thanks.
making mRNA, I run a simple agarose gel. There are two bands. One band is
not very close to the actual size (400bp smaller), there is also another
smaller and stronger band. Can I still use the mRNA? Why there are two bands
? Thanks.
c*7
4 楼
看你能从naf还是nih拿grant 了
【在 j******3 的大作中提到】
: biotech方向 哪个学院更好？除了soft/hard money的区别以外 其他有没有特别的不同
: ？medical school是不是funding压力会很大?
【在 j******3 的大作中提到】
: biotech方向 哪个学院更好？除了soft/hard money的区别以外 其他有没有特别的不同
: ？medical school是不是funding压力会很大?
s*h
5 楼
EB-1B 能DIY吗？我的影响是必须找lawyer.
x*w
6 楼
Did you purify mRNA before electrophoresis?
Un-incorporated NTP also could be detected by EB.
Un-incorporated NTP also could be detected by EB.
w*y
7 楼
不知道答案，但是帮你顶一下。如果成功了请一定告诉大家一下经验！
140应该不需要单位签字了，因为你是efile，suppose 应该是单位来file, 是internet
agree.
140应该不需要单位签字了，因为你是efile，suppose 应该是单位来file, 是internet
agree.
h*g
8 楼
I did not purify mRNA. Could you tell me how to purify it? Using what kit?
Thanks!
【在 x**w 的大作中提到】
: Did you purify mRNA before electrophoresis?
: Un-incorporated NTP also could be detected by EB.
Thanks!
【在 x**w 的大作中提到】
: Did you purify mRNA before electrophoresis?
: Un-incorporated NTP also could be detected by EB.
r*n
9 楼
Did you use denature gel? RNA gel? Normal DNA gels may not give you good
estimation on the size. Purification I used RNAeasy kit from Qiagen, works
well. You could use classic pheno-chloroform extraction if you don't have
the kit. If your RNA coded product has fluorescence or very specific
phenotypes in your system maybe a direct functional verification is easier.
good luck
estimation on the size. Purification I used RNAeasy kit from Qiagen, works
well. You could use classic pheno-chloroform extraction if you don't have
the kit. If your RNA coded product has fluorescence or very specific
phenotypes in your system maybe a direct functional verification is easier.
good luck
c*r
10 楼
mMESSENGER mMACHINE 说明书里提供了好像是三种纯化方法。试剂盒里也有一些纯化所
需的试剂。
【在 h*********g 的大作中提到】
: I did not purify mRNA. Could you tell me how to purify it? Using what kit?
: Thanks!
需的试剂。
【在 h*********g 的大作中提到】
: I did not purify mRNA. Could you tell me how to purify it? Using what kit?
: Thanks!
x*w
11 楼
My experience is LiCl +isopropanol works perfect.
h*g
12 楼
Thanks. I used normal DNA gel. I don't know why there two bands. Thanks.
【在 r********n 的大作中提到】
: Did you use denature gel? RNA gel? Normal DNA gels may not give you good
: estimation on the size. Purification I used RNAeasy kit from Qiagen, works
: well. You could use classic pheno-chloroform extraction if you don't have
: the kit. If your RNA coded product has fluorescence or very specific
: phenotypes in your system maybe a direct functional verification is easier.
: good luck
【在 r********n 的大作中提到】
: Did you use denature gel? RNA gel? Normal DNA gels may not give you good
: estimation on the size. Purification I used RNAeasy kit from Qiagen, works
: well. You could use classic pheno-chloroform extraction if you don't have
: the kit. If your RNA coded product has fluorescence or very specific
: phenotypes in your system maybe a direct functional verification is easier.
: good luck
s*n
13 楼
Did you digest the DNA template? Did you run a formaldehyde gel? Did you use
RNA ladder?
RNA runs at lower MW comparing to dsDNA; if you are not using RNA gel, your
RNA will be easily degraded.
RNA ladder?
RNA runs at lower MW comparing to dsDNA; if you are not using RNA gel, your
RNA will be easily degraded.
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