请教，有什么小分子和protein（monomer）的结合强度可以接近biotin和 streptavidin?# Biology - 生物学
s*e
1 楼
最近优酷内嵌播放有些问题，如果无法正常播放请通过这个链接观看：
http://v.youku.com/v_show/id_XNzk4MjM3MzM2.html
http://v.youku.com/v_show/id_XNzk4MjM3MzM2.html
c*u
2 楼
谢谢 各位提供线索啊
S*s
3 楼
厉害！
c*u
4 楼
streptavidin是多聚物 实在没有办法用。
提前谢谢提供线索的各位啊
提前谢谢提供线索的各位啊
a*g
5 楼
不错。
【在 s******e 的大作中提到】
: 最近优酷内嵌播放有些问题，如果无法正常播放请通过这个链接观看：
: http://v.youku.com/v_show/id_XNzk4MjM3MzM2.html
【在 s******e 的大作中提到】
: 最近优酷内嵌播放有些问题，如果无法正常播放请通过这个链接观看：
: http://v.youku.com/v_show/id_XNzk4MjM3MzM2.html
s*x
6 楼
如果是要化学label的话有很多类似click chemistry的方法。
如果只是结合，好像halotag有小分子可以bind。但是都不普及，不知道work的怎么样。
如果只是结合，好像halotag有小分子可以bind。但是都不普及，不知道work的怎么样。
c*u
7 楼
非常感谢 已经再化学版 提问了
样。
【在 s********x 的大作中提到】
: 如果是要化学label的话有很多类似click chemistry的方法。
: 如果只是结合，好像halotag有小分子可以bind。但是都不普及，不知道work的怎么样。
样。
【在 s********x 的大作中提到】
: 如果是要化学label的话有很多类似click chemistry的方法。
: 如果只是结合，好像halotag有小分子可以bind。但是都不普及，不知道work的怎么样。
A*y
8 楼
Not possible unless you form a specific covalent bond, which will depend on
your protein. There are some very specific covalent inhibitors.
your protein. There are some very specific covalent inhibitors.
M*g
9 楼
you can try SNAP tag https://www.neb.com/tools-and-resources/feature-
articles/snap-tag-technologies-novel-tools-to-study-protein-function) with
BG substrates https://www.neb.com/products/s9151-bg-gla-nhs or https://www.
neb.com/products/s9153-bg-maleimide).
BG is a small molecule and it will form covalent bond with SNAP protein.
BG can be modified with GLA-NHS group (primary amine reactive) or maleimide
(-SH group reactive). So BG-GLA-NHS or BG-maleimide can be attach to a
protein via primary amine or SH group, and SNAP will then react with BG to
form a covalent bond.
Hope this helps.
articles/snap-tag-technologies-novel-tools-to-study-protein-function) with
BG substrates https://www.neb.com/products/s9151-bg-gla-nhs or https://www.
neb.com/products/s9153-bg-maleimide).
BG is a small molecule and it will form covalent bond with SNAP protein.
BG can be modified with GLA-NHS group (primary amine reactive) or maleimide
(-SH group reactive). So BG-GLA-NHS or BG-maleimide can be attach to a
protein via primary amine or SH group, and SNAP will then react with BG to
form a covalent bond.
Hope this helps.
c*u
10 楼
非常感谢
on
【在 A******y 的大作中提到】
: Not possible unless you form a specific covalent bond, which will depend on
: your protein. There are some very specific covalent inhibitors.
on
【在 A******y 的大作中提到】
: Not possible unless you form a specific covalent bond, which will depend on
: your protein. There are some very specific covalent inhibitors.
c*u
11 楼
嗯 感谢这么详细的答复
www.
maleimide
【在 M******g 的大作中提到】
: you can try SNAP tag https://www.neb.com/tools-and-resources/feature-
: articles/snap-tag-technologies-novel-tools-to-study-protein-function) with
: BG substrates https://www.neb.com/products/s9151-bg-gla-nhs or https://www.
: neb.com/products/s9153-bg-maleimide).
: BG is a small molecule and it will form covalent bond with SNAP protein.
: BG can be modified with GLA-NHS group (primary amine reactive) or maleimide
: (-SH group reactive). So BG-GLA-NHS or BG-maleimide can be attach to a
: protein via primary amine or SH group, and SNAP will then react with BG to
: form a covalent bond.
: Hope this helps.
www.
maleimide
【在 M******g 的大作中提到】
: you can try SNAP tag https://www.neb.com/tools-and-resources/feature-
: articles/snap-tag-technologies-novel-tools-to-study-protein-function) with
: BG substrates https://www.neb.com/products/s9151-bg-gla-nhs or https://www.
: neb.com/products/s9153-bg-maleimide).
: BG is a small molecule and it will form covalent bond with SNAP protein.
: BG can be modified with GLA-NHS group (primary amine reactive) or maleimide
: (-SH group reactive). So BG-GLA-NHS or BG-maleimide can be attach to a
: protein via primary amine or SH group, and SNAP will then react with BG to
: form a covalent bond.
: Hope this helps.
f*u
12 楼
印象里好象有monomeric streptavidin吧，也许对你来说可以用？
M*g
13 楼
kd for monomeric streptavidin is high, 10e-9, as compared to the kd of
tetrameric streptavidin, < 10e-12
tetrameric streptavidin, < 10e-12
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