a*e
2 楼
【 以下文字转载自 Parenting 讨论区 】
发信人: ac2624 (Chris), 信区: Parenting
标 题: 真着急!小孩才移民过来,基本不会英语,我怕得自闭症。
发信站: BBS 未名空间站 (Mon Jul 25 01:59:26 2011, 美东)
因为工作关系,我们一家移民过来美国,小孩已经11岁了,不放心把小孩放在国内,所
以就一同带来了。没想到因为语言的关系,我家小孩基本上不能与外界交流,学校的老
师也反应我家小孩在学校里面很孤僻。怎么办怎么办??现在正是小孩心理和生理成长
的重要阶段!我十分担心我小孩以后性格的塑造。请问有没有与我类似并且有成功经验
的网友,能帮我解决这个头疼的问题??
发信人: ac2624 (Chris), 信区: Parenting
标 题: 真着急!小孩才移民过来,基本不会英语,我怕得自闭症。
发信站: BBS 未名空间站 (Mon Jul 25 01:59:26 2011, 美东)
因为工作关系,我们一家移民过来美国,小孩已经11岁了,不放心把小孩放在国内,所
以就一同带来了。没想到因为语言的关系,我家小孩基本上不能与外界交流,学校的老
师也反应我家小孩在学校里面很孤僻。怎么办怎么办??现在正是小孩心理和生理成长
的重要阶段!我十分担心我小孩以后性格的塑造。请问有没有与我类似并且有成功经验
的网友,能帮我解决这个头疼的问题??
r*e
3 楼
I don't know anyone here has experience with plasmid transfection fir SH-
SY5Y?
Lipofectamine 3000 doesn't work at all.
Neuronal line is always challenging for transfection.
Any recommendations of good reagent to achieve relatively high transfection
efficiency?
Thx!
SY5Y?
Lipofectamine 3000 doesn't work at all.
Neuronal line is always challenging for transfection.
Any recommendations of good reagent to achieve relatively high transfection
efficiency?
Thx!
y*t
5 楼
j point?
d*n
6 楼
据说吐口水有奇效
j*9
8 楼
I have tried about 10 lipid-based transfection reagents in SH-SY5Y and
neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
Good luck
transfection
【在 r**********e 的大作中提到】
: I don't know anyone here has experience with plasmid transfection fir SH-
: SY5Y?
: Lipofectamine 3000 doesn't work at all.
: Neuronal line is always challenging for transfection.
: Any recommendations of good reagent to achieve relatively high transfection
: efficiency?
: Thx!
neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
Good luck
transfection
【在 r**********e 的大作中提到】
: I don't know anyone here has experience with plasmid transfection fir SH-
: SY5Y?
: Lipofectamine 3000 doesn't work at all.
: Neuronal line is always challenging for transfection.
: Any recommendations of good reagent to achieve relatively high transfection
: efficiency?
: Thx!
U*e
10 楼
我们公司自己开发了一种转染试剂,同现在市售的转染试剂都不同,比较广谱,相对各
种核酸及细胞都可以用。而且可以用荧光实时检测转染试剂同质粒符合物的生成及转染
细胞的过程。产品还没用放入公司网页。如果你想试试,我们可以提供试用样品。
Bioandbeyond.com
种核酸及细胞都可以用。而且可以用荧光实时检测转染试剂同质粒符合物的生成及转染
细胞的过程。产品还没用放入公司网页。如果你想试试,我们可以提供试用样品。
Bioandbeyond.com
n*d
11 楼
长得象这样, medium hot都行
http://www.youboy.com/pics60178991.html
http://www.youboy.com/pics60178991.html
c*i
12 楼
我们lab用FuGene HD,效率一般。现在准备做lenti
k*s
13 楼
walmart的 big birtha,我喜欢,不辣,皮很薄
c*s
14 楼
Xfect from Clontech is relatively the best in our hand.
transfection
【在 r**********e 的大作中提到】
: I don't know anyone here has experience with plasmid transfection fir SH-
: SY5Y?
: Lipofectamine 3000 doesn't work at all.
: Neuronal line is always challenging for transfection.
: Any recommendations of good reagent to achieve relatively high transfection
: efficiency?
: Thx!
transfection
【在 r**********e 的大作中提到】
: I don't know anyone here has experience with plasmid transfection fir SH-
: SY5Y?
: Lipofectamine 3000 doesn't work at all.
: Neuronal line is always challenging for transfection.
: Any recommendations of good reagent to achieve relatively high transfection
: efficiency?
: Thx!
n*d
15 楼
谢谢各位推荐
s*g
16 楼
lonza电转
即使用其他公司试剂(Mirus)也行
我曾经也面对过难转染的细胞
即使用其他公司试剂(Mirus)也行
我曾经也面对过难转染的细胞
l*d
17 楼
很像我以前种过的杭椒。
不过辣椒的辣度跟光照也有关系,我种的jalapeno,秋天了就不是太辣了。
【在 n*******d 的大作中提到】
: 长得象这样, medium hot都行
: http://www.youboy.com/pics60178991.html
不过辣椒的辣度跟光照也有关系,我种的jalapeno,秋天了就不是太辣了。
【在 n*******d 的大作中提到】
: 长得象这样, medium hot都行
: http://www.youboy.com/pics60178991.html
U*e
18 楼
Updated link: visible transfection
http://bioandbeyond.com/p/whats-new
http://bioandbeyond.com/p/whats-new
r*e
20 楼
Hi Thanks
For NeuroPORTER
Genlantis or Sigma-Aldrich, which one is better? And why the same product
but two companies? And I don't see Genlantis is being merged by Sigma
or
【在 j*****9 的大作中提到】
: I have tried about 10 lipid-based transfection reagents in SH-SY5Y and
: neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
: Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
: Good luck
:
: transfection
For NeuroPORTER
Genlantis or Sigma-Aldrich, which one is better? And why the same product
but two companies? And I don't see Genlantis is being merged by Sigma
or
【在 j*****9 的大作中提到】
: I have tried about 10 lipid-based transfection reagents in SH-SY5Y and
: neurons. None of them gave me a decent efficiency. NeuroPORTER (Genlantis or
: Sigma-Aldrich) was the best (~30% in SH-SY5Y, 18% in Neurons using FACS).
: Good luck
:
: transfection
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