I just developed a new method using LC/MS/MS, but when injecting the same standards (tried same vial and different vials), the peak area is very different from run to run, also the peak shape becomes horrible after a few injections. Anyone knows any possible reasons? Thanks!
I tested another method, and injected the same solution several times, and it was fine. I switched to another column today. Would the column also contribute to the intensity difference?
same
【在 G***O 的大作中提到】 : 把方法和样品的情况说明一下。我估计是不是样品把柱子搞坏了。 : : :I just developed a new method using LC/MS/MS, but when injecting the same : :standards (tried same vial and different vials), the peak area is very
Can you try positive mode with a different method that has been proved working before? Matrix effect or your column is dirty. If you have charging effect on your quadruple, just run the tuning solution at positive mode for a while, your TIC will start high and then tail down significantly. It wouldn't stay as a straight line.