Re: Southern Blot问题求教！# Biology - 生物学
M*e
1 楼
for genomice southern, you should have very good quality DNA
and digest it completely. it is better to purify the gDNA
and remove anything that may inhibit digestion. typically,
i would digest about 10-20ug of gDNA O/N with enough enzyme
and add another shot next day. it is not suggested to add
EtBr into the gel, though i found it was fine with mine.
you should follow a good protocol that is known to work for
genomic southern. my opinion is that transfer should be very
good. after depurination a
and digest it completely. it is better to purify the gDNA
and remove anything that may inhibit digestion. typically,
i would digest about 10-20ug of gDNA O/N with enough enzyme
and add another shot next day. it is not suggested to add
EtBr into the gel, though i found it was fine with mine.
you should follow a good protocol that is known to work for
genomic southern. my opinion is that transfer should be very
good. after depurination a