Re: Kozak sequence# Biology - 生物学
e*e
1 楼
Perfect Kozak is not as short as CCACC.
In your case, if upstream of the CACC is a G instead of A, downstream of ATG
is also a G, it maybe works (less) efficiently; otherwise, I would reconstruct
again.
Perfect Kozak is not as short as CCACC.
In your case, if upstream of the CACC is a G instead of A, downstream of ATG
is also a G, it maybe works (less) efficiently; otherwise, I would reconstruct
again.
g*r
2 楼
Kozak
Sequence for
Mammalian
Expression
If you will be recombining your entry clone with a
destination vector for mammalian expression, your insert
should contain a Kozak consensus sequence with an ATG
initiation codon for proper initiation of translation (Kozak,
1987; Kozak, 1991; Kozak, 1990). An example of a Kozak
consensus sequence is provided below. Other sequences are
possible, but the G or A at position -3 and the G at position
+4 are the most critical for function (shown in bold). The
AT
Sequence for
Mammalian
Expression
If you will be recombining your entry clone with a
destination vector for mammalian expression, your insert
should contain a Kozak consensus sequence with an ATG
initiation codon for proper initiation of translation (Kozak,
1987; Kozak, 1991; Kozak, 1990). An example of a Kozak
consensus sequence is provided below. Other sequences are
possible, but the G or A at position -3 and the G at position
+4 are the most critical for function (shown in bold). The
AT
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