Re: 熟悉果蝇的看过来，有个问题要请教# Biology - 生物学
a*r
1 楼
A gene causing lethality suggests it might be a functionally important. But a
second hit in the genome might contribute the lethal phenotype. This is why
some labs clean the background of P element lines before they make the
imprecise hopout. If you can get sevearl deficiency lines or the mutant allele
for the neighboring gene, try several complementaion tests.
Check the expression pattern and functional domains for you gene, that might
help you to figure out its possible role. Making a UAS line
second hit in the genome might contribute the lethal phenotype. This is why
some labs clean the background of P element lines before they make the
imprecise hopout. If you can get sevearl deficiency lines or the mutant allele
for the neighboring gene, try several complementaion tests.
Check the expression pattern and functional domains for you gene, that might
help you to figure out its possible role. Making a UAS line