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已经ko 的蛋白质 什么情况下又表达了?
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已经ko 的蛋白质 什么情况下又表达了?# Biology - 生物学
x*n
1
我父母的09年9月中旬拿到的签证,准备赶在deadline之前中信代签,不知道可以不可以呢。
我父亲来了一次,我母亲二次入境,每次呆五个多月。就怕被拒。
我之前听说代签要邀请信,如果需要,是提供我们给父母的邀请信呢,还是我给签证官的表明我们邀请父母来的信呢?
还有就是我刚f2转成f1,这会不会有影响?签证官那儿有没有纪录的?
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l*n
2
正在研究行星互容,看到 Tyl 的这篇分析,感觉很生动,豁然开朗不少
The Subtle Powers of Mutual Reception
“Mutual Reception” reminds me of time-share house-swaps: you have an
apartment in New York City and want to summer in Florence; someone in
Florence wants to come to New York at the same time. The deal is made, you
live in each other’s domain, getting very close together without ever
meeting, and the cooperation touches both lives.
It also reminds me of a magician and his assistant: the performer and his
helper sharing a highly integrated performing rapport; the former is seen,
the latter need not be, and the effect is remarkable!
Mutual Reception in astrology occurs when two planets relate by the fact
that each is in the sign ruled by the other; that’s time-share swap. And
when the two planets in each other’s domain are ALSO in aspect, the wedding
of energies can be magical dramatic.
For example, the great actress/singer Cher (May 20, 1946 at 7:31 AM PST, El
Centro CA) has a Cancer Ascendant with Saturn in Cancer in the first House.
The Moon is in Capricorn, ruled by Saturn, in the 7th House, and the two
planets, besides being in mutual reception, are also in opposition to each
other.
This is an extremely powerful statement in the horoscope. I would synthesize
it as “finding the Self (the Moon, ruling the Ascendant) through others (
Saturn ruling the 7th).” This highly focused statement crashes into the
horoscope through the door opened by Neptune in the 4th, squaring the
Ascendant: confusion, bewilderment, in the early home [Cher’s father
abandoned the family the day Cher was born]. Father surrogate outreach would
have dominated Cher’s early years of development and begun in earnest with
the arrival of Sonny Bono, much her senior, when she was 16 years old.
Michael Jackson’s (August 29, 1958 at 11:53 PM CDT in Gary , IN) Sun is in
Virgo and Mercury-retrograde is in Leo. The two are in mutual reception but
not in aspect. Each will reach to fuse with the other (a seasoning of
idealism), and both will do so in terms of the House(s) involved by location
and rulership.
The two planets occur along with Pluto, which is conjunct the Sun, in the
4th House, suggesting a major suppression and frustration within the early
home. This could be relegated to the contrapuntal retrogradation complexity
of Mercury. Mercury rules the 5th, introducing the concept of childhood,
children, part of the sex profile. –Mercury also rules Jackson’s Gemini
Ascendant; this mutual reception influence involving the 4th House, the 5th,
and the Ascendant, the mind and life energy in development out of the early
home sing a powerful idealized song of hope in his make-up.
Prince Charles (November 14, 1948 at 9:14 PM in London, ENG) has the
powerful, sub-generational mutual reception between Pluto and the Sun –
Pluto in Leo and the Sun in Scorpio—with the two in square aspect.
We feel a tremendous suppression of the powers to be. There is a blanket
over Charles’ hand-grenade. The lid is tight on his pressure cooker. –With
the Sun ruling his Ascendant, Pluto in the Ascendant, ruling the 5th, we
readily suspect the focus of his frustrations in terms of loving another,
being himself securely and openly (Neptune-Venus are on the IC!).
Actor Tom Cruise (July 3, 1962 at 3:06 PM EDT in Syracuse NY) has the Sun
in Cancer and the Moon in Leo: a definite self-dramatization, acting out
needs for appreciation and love. There can be a show of “rightness” that
helps with understanding and acceptance by others. There’s an impression of
pride and self-confidence established, and others believe it.
For Cruise, the Sun rules the Midheaven and provides the much needed anchor
here for the peregrine Moon (dissociation complex), unable to fit in easily
anywhere, constantly on the move, feeling lost. The Sun is in a water grand
trine with Neptune and Jupiter: a closed-circuit of emotional self-
sufficiency, which is led by rulership by the Sun of the Midheaven directly
into career development. Cruise is his vocation. It defines him. It saves
him.
Brilliant comedian, director, and philanthropist Jerry Lewis (March 16, 1926
at 12:15 PM EST in Newark NJ) has Sun and Neptune in mutual reception, but
not in aspect. The Sun is intensified by the exact conjunction with Uranus,
and the Sun rules the financial 2nd. –Neptune, in the 2nd ,with all its
theatricality (Neptune=MC/Asc), rules the Midheaven. The mutual reception
between Sun and Neptune links all of their experiential references together.
Toweringly celebrated evangelist Billy Graham (November 7, 1918 at 3:30 PM
EST in Charlotte NC) has the Moon in Sagittarius, conjunct the Midheaven and
Mars with Jupiter in Cancer quindecile the Midheaven, ruling the 9th House
and trine the Sun! --No more need be said about the link of lofty
opinionation, internationalism, education, and profession!
Sarah Ferguson (October 15, 1959 at 9:03 AM GMT in London ENG) has Mercury
and Pluto in mutual reception and sextile. Mercury rules the MC and Pluto on
the Midheaven (exhibitionism) rules the Ascendant. This links very, very
strongly, the establishment of personal identity with the formulation of a
major career. Ferguson could no more be kept hidden behind a stifling royal
marriage than a bull could be kept out of a china shop!
I wish I had a dollar for every time I said, “I live in my Venus-Uranus
quintile”! This creative measurement is so very strong because Venus is in
Aquarius and Uranus is in Taurus; the two are in tight quintile aspect and
also are in mutual reception! (Noel Tyl: December 31 at 3:57 PM EST in West
Chester PA).
Uranus rules the 9th where Venus is located: I have published 30 books,
lectured throughout 18 countries, and had long relationships with several
foreign-born ladies. –Venus rules the 5th .
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E*C
3
最近看的文章,已经敲掉的蛋白质 ,肿瘤种植之后,又表达了
咋回事涅
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r*a
4
可以代签,邀请信可要可不要,我们第一次代签给了信,第二次给了没有收
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d*c
5
多谢分享!
挺subtle的:上面Cher的例子月亮土星其实都处在失势位置,但mutual reception并且
是冲相。
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s*y
6
可以有很多种情况啊。
比方说他们敲的时候只是敲掉了第一个exon,但是其他exon东剪切西剪切之后
在某个条件下又剪出一个有活性的truncate了

【在 E*C 的大作中提到】
: 最近看的文章,已经敲掉的蛋白质 ,肿瘤种植之后,又表达了
: 咋回事涅

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m*n
7
可以在签证过期前找中信代签?
你说的时间是不是应该是2010年9月拿到签证的
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d*c
8
有几个问题:
为什么mutual reception考虑的是入庙关系而不是入旺,比如"by definition", 水星
在金牛同时金星在水瓶并不是mutual reception?
比如水瓶座,采用守护星土星(古典)还是天王星(现代)?
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o*4
9
我晕

【在 s******y 的大作中提到】
: 可以有很多种情况啊。
: 比方说他们敲的时候只是敲掉了第一个exon,但是其他exon东剪切西剪切之后
: 在某个条件下又剪出一个有活性的truncate了

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HP
10
过期不超一年都可以中信代签吧
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l*n
11
rulership and exaltation
入庙,入旺都可以
定义是:
http://www.astrologyweekly.com/dictionary/mutual-reception.php
是一种对应,A 在 B 家, B 在A 家,两颗行星,同时交换房子住,
前提是,两颗星,必须是同一“级别”的,而且同时交换。
这是一种互相引导和交流的关系,positive 的作用。
对于同时有两颗守护星的星座, 摩羯,水瓶,双鱼,两颗守护星都可以。
因为,任何一张盘,该盘的任一课行星,肯定只能落在一个星座。

【在 d****c 的大作中提到】
: 有几个问题:
: 为什么mutual reception考虑的是入庙关系而不是入旺,比如"by definition", 水星
: 在金牛同时金星在水瓶并不是mutual reception?
: 比如水瓶座,采用守护星土星(古典)还是天王星(现代)?

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s*y
12
别晕。这种情况不少见。因为很多alternative splicing 并不需要第一个exon

【在 o**4 的大作中提到】
: 我晕
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l*n
13
这篇也是分析 互容的
核心就是,看到盘里有互容的情况,一定两颗星一起分析,然后再分析两个星直接的力
量对比。
我觉得或许任何一种情况(庙弱旺陷)都可以,只要两颗星是同一级别。
http://www.alanoken.com/newsletter/Dec07/newsletter1207d.html

【在 d****c 的大作中提到】
: 有几个问题:
: 为什么mutual reception考虑的是入庙关系而不是入旺,比如"by definition", 水星
: 在金牛同时金星在水瓶并不是mutual reception?
: 比如水瓶座,采用守护星土星(古典)还是天王星(现代)?

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o*4
14
一般ko,都会把最重要的exon给敲掉吧

【在 s******y 的大作中提到】
: 别晕。这种情况不少见。因为很多alternative splicing 并不需要第一个exon
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d*c
15
从统计角度来看,我觉得超过1/3的盘是有互容现象的。
对了,你有研究过中点,ASC与MC中点说明了啥?谢谢。
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s*y
16
可是有时候我们的知识并不是那么完善,敲的时候根据理论以为是最重要的
exon并不一定就真的是细胞里最重要的exon, 甚至有些隐藏的exon很多人
并不一定就知道。
所以敲完之后,必须要用northern blot and polyclonal ab WB 做验证,就是这个原因

【在 o**4 的大作中提到】
: 一般ko,都会把最重要的exon给敲掉吧
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d*c
17
很感谢,请教下
除了Sue Tompkins的书,还有什么你特别非常推荐的吗,针对星盘分析?
并祝新春快乐!

【在 l*****n 的大作中提到】
: rulership and exaltation
: 入庙,入旺都可以
: 定义是:
: http://www.astrologyweekly.com/dictionary/mutual-reception.php
: 是一种对应,A 在 B 家, B 在A 家,两颗行星,同时交换房子住,
: 前提是,两颗星,必须是同一“级别”的,而且同时交换。
: 这是一种互相引导和交流的关系,positive 的作用。
: 对于同时有两颗守护星的星座, 摩羯,水瓶,双鱼,两颗守护星都可以。
: 因为,任何一张盘,该盘的任一课行星,肯定只能落在一个星座。

avatar
o*4
18
体内确实太复杂了,人算不如天算;
southern blot验证有啥用?基因组没问题了,蛋白质有可能还是会被western检测到吧

原因

【在 s******y 的大作中提到】
: 可是有时候我们的知识并不是那么完善,敲的时候根据理论以为是最重要的
: exon并不一定就真的是细胞里最重要的exon, 甚至有些隐藏的exon很多人
: 并不一定就知道。
: 所以敲完之后,必须要用northern blot and polyclonal ab WB 做验证,就是这个原因

avatar
l*n
19
有的,貌似tyl 分析太阳弧都会用中点理论的。
哪颗星落在 asc mc 的中点位置?
我全部贴给你吧
MC/AS=SO To have close professional contact with others. Working together.
MC/SO=AS The mental and professional attitude toward others.
AS/SO=MC Personal conduct toward other people. The impression made by the
personal environment on the native.
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s*y
20
常规验证,双保险,万一出了问题,也可以定点排除看看到底是那一环出了问题

【在 o**4 的大作中提到】
: 体内确实太复杂了,人算不如天算;
: southern blot验证有啥用?基因组没问题了,蛋白质有可能还是会被western检测到吧
:
: 原因

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G*r
21
月MM简直是百科全书啊,太赞了~

you
his
seen,

【在 l*****n 的大作中提到】
: 正在研究行星互容,看到 Tyl 的这篇分析,感觉很生动,豁然开朗不少
: The Subtle Powers of Mutual Reception
: “Mutual Reception” reminds me of time-share house-swaps: you have an
: apartment in New York City and want to summer in Florence; someone in
: Florence wants to come to New York at the same time. The deal is made, you
: live in each other’s domain, getting very close together without ever
: meeting, and the cooperation touches both lives.
: It also reminds me of a magician and his assistant: the performer and his
: helper sharing a highly integrated performing rapport; the former is seen,
: the latter need not be, and the effect is remarkable!

avatar
o*4
22
southern只是验证genome,不能保证剪切的问题吧,呵呵

【在 s******y 的大作中提到】
: 常规验证,双保险,万一出了问题,也可以定点排除看看到底是那一环出了问题
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l*n
23
符号,我注一下,其实估计不注你也看得明白的 :)
sol :sun
mo: moon
me:mercury
ve: venus
ma: mars
ju: jupiter
sa: saturn
后面三个分别就是天海冥。。。

【在 l*****n 的大作中提到】
: 有的,貌似tyl 分析太阳弧都会用中点理论的。
: 哪颗星落在 asc mc 的中点位置?
: 我全部贴给你吧
: MC/AS=SO To have close professional contact with others. Working together.
: MC/SO=AS The mental and professional attitude toward others.
: AS/SO=MC Personal conduct toward other people. The impression made by the
: personal environment on the native.

avatar
s*y
24
设计得好的也可以啊,比方说如果是切5-end exon, 那就用3-end的探针来杂交,
看看转录出来的片断有多长。如果出来一条不是在意料之中的长度的话,
那就说明可能出来了意外的剪切

【在 o**4 的大作中提到】
: southern只是验证genome,不能保证剪切的问题吧,呵呵
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d*c
25
灰常感谢!
找到了,我现在关心
MC/AS=MO One's behavior toward women. Professional relations to the public
and women.

【在 l*****n 的大作中提到】
: 符号,我注一下,其实估计不注你也看得明白的 :)
: sol :sun
: mo: moon
: me:mercury
: ve: venus
: ma: mars
: ju: jupiter
: sa: saturn
: 后面三个分别就是天海冥。。。

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p*d
26
也有可能是少部分细胞ko效率不好,成为优势群,形成肿瘤。或者promoter失活也是可
能的。
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l*n
27
偶们大家一起来专研,吼吼~~

【在 G****r 的大作中提到】
: 月MM简直是百科全书啊,太赞了~
:
: you
: his
: seen,

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o*4
28
你说的是nothern?

【在 s******y 的大作中提到】
: 设计得好的也可以啊,比方说如果是切5-end exon, 那就用3-end的探针来杂交,
: 看看转录出来的片断有多长。如果出来一条不是在意料之中的长度的话,
: 那就说明可能出来了意外的剪切

avatar
l*n
29
有的, Noel Tyl 的 有本红宝书
Sythesis & Counseling in Astrology
里面大篇幅的星盘案例分析
还有, Stephen Arroyo 的书也是经典
比如这本
http://www.cafeastrology.com/reviews/arroyochartinterpretation.

【在 d****c 的大作中提到】
: 很感谢,请教下
: 除了Sue Tompkins的书,还有什么你特别非常推荐的吗,针对星盘分析?
: 并祝新春快乐!

avatar
a*k
30
能问一下为啥要用southern而不用PCR么?就是平时做genotyping用的,为啥southern
这样麻烦的东西用得很多呢?

原因

【在 s******y 的大作中提到】
: 可是有时候我们的知识并不是那么完善,敲的时候根据理论以为是最重要的
: exon并不一定就真的是细胞里最重要的exon, 甚至有些隐藏的exon很多人
: 并不一定就知道。
: 所以敲完之后,必须要用northern blot and polyclonal ab WB 做验证,就是这个原因

avatar
d*c
31
恩,Stephen Arroyo好像是瓶子,
瓶子的书我真的蛮喜欢!

【在 l*****n 的大作中提到】
: 有的, Noel Tyl 的 有本红宝书
: Sythesis & Counseling in Astrology
: 里面大篇幅的星盘案例分析
: 还有, Stephen Arroyo 的书也是经典
: 比如这本
: http://www.cafeastrology.com/reviews/arroyochartinterpretation.

avatar
s*y
32
Yeah! Sorry my mistake!

【在 o**4 的大作中提到】
: 你说的是nothern?
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d*c
33
Stephen Arroyo是上升水瓶。
Have to flatter you: 第三次out of三次赞你的形象!
来自Venus,毫无疑问啦

【在 l*****n 的大作中提到】
: 有的, Noel Tyl 的 有本红宝书
: Sythesis & Counseling in Astrology
: 里面大篇幅的星盘案例分析
: 还有, Stephen Arroyo 的书也是经典
: 比如这本
: http://www.cafeastrology.com/reviews/arroyochartinterpretation.

avatar
p*m
34
我猜她说的是northern,这样才谈得上看什么3'转录的问题 对于KO来说看看northern
,或者对于转基因来说看看southern,而不光是PCR 主要还是考虑到KO可能会在recombination的时候发生了怪异的重组 甚至把切出来的片段插到别处去了 这一步因为有个open end在那里飘着 可能会发生没法预测的事情。transgene也一样,transgene还存在个copy number问题

southern

【在 a********k 的大作中提到】
: 能问一下为啥要用southern而不用PCR么?就是平时做genotyping用的,为啥southern
: 这样麻烦的东西用得很多呢?
:
: 原因

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w*y
35
看来MM已经搞定上次我问你的关于中点的问题了
问个题外的,宿命点和福点的相位影响大吗?尤其对于出生图中相位比例大

【在 l*****n 的大作中提到】
: 有的,貌似tyl 分析太阳弧都会用中点理论的。
: 哪颗星落在 asc mc 的中点位置?
: 我全部贴给你吧
: MC/AS=SO To have close professional contact with others. Working together.
: MC/SO=AS The mental and professional attitude toward others.
: AS/SO=MC Personal conduct toward other people. The impression made by the
: personal environment on the native.

avatar
s*y
36
Northern blot, Northern blot. Sorry my mistake :)
The good thing of northern is that you can directly see the real size of the
mRNA, which is not very doable with PCR

southern

【在 a********k 的大作中提到】
: 能问一下为啥要用southern而不用PCR么?就是平时做genotyping用的,为啥southern
: 这样麻烦的东西用得很多呢?
:
: 原因

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c*r
37
有几种情况:
1. 上面sunny同学说的很对。有alternative splicing等其他可能。
2. ko一般都是ko一两个exons,一般assume不完整的RNA或者peptide不稳定,会很快被
代谢掉。但是事实上经常不是这样。有很多蛋白即使ko掉几个exons后还有detectable
的peptide,虽然size shift了,但是确实western可以detect。
3. 如果是conditional ko就有ko效率,和部位,细胞种类等其他原因导致在一定程度
上可以detectable。
不论怎样,要证明ko都要从几个方面来证明:
1. 从genomic上,要跑southern,看到size减小as expected。
2. 从RNA上,northern,和western证明没有detectable的stable的蛋白。如果有,要
证明这个蛋白的功能被comprise了。
3. 从功能上证明这个蛋白的功能没有了。
4. 从phenotype上证明这个功能和这个phenotype直接的联系。
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H*N
38
Which paper?
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f*e
39
KO 有很多种:Conventioanl KO,可能会出现truncate
conditional 一旦cre表达不全或不过 确实可能KO不全
inducible的那就更有可能了
移植的肿瘤后有表达?
是移植了KO的肿瘤还是一直到KO的小鼠,这样两种细胞有一种不是KO就嘛事就有可能了
啊?

【在 E*C 的大作中提到】
: 最近看的文章,已经敲掉的蛋白质 ,肿瘤种植之后,又表达了
: 咋回事涅

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