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如何解释不同受体表达的时间差异
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如何解释不同受体表达的时间差异# Biology - 生物学
m*2
1
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LC-ONLCHK4Q11
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f*s
2
我做的一个化合物在老鼠感染后第1,5天抑制tlr4,但是在7天抑制tlr1,在1,5,7天抑制tlr6,
在7天抑制tlr8. reviewer问如何解释在不同天数的表达的差异?如何解释这里没有
kinetics pattern as when these changes will occur?这些数据就是这样的结果,
也许当时调整一下数据会得到更好的结果方便解释,可是我保留了较为原始的数据。不
知该如何解释?
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f*r
3
老鼠里有TLR8?

制tlr6,

【在 f******s 的大作中提到】
: 我做的一个化合物在老鼠感染后第1,5天抑制tlr4,但是在7天抑制tlr1,在1,5,7天抑制tlr6,
: 在7天抑制tlr8. reviewer问如何解释在不同天数的表达的差异?如何解释这里没有
: kinetics pattern as when these changes will occur?这些数据就是这样的结果,
: 也许当时调整一下数据会得到更好的结果方便解释,可是我保留了较为原始的数据。不
: 知该如何解释?

avatar
a*g
4
If you don't need experiments to support your explanation, you can just say
the compound might be metabolized in liver during the period of
experimentation. The different metabolites of your compound may account for
the differential activity towards TLRs.

制tlr6,

【在 f******s 的大作中提到】
: 我做的一个化合物在老鼠感染后第1,5天抑制tlr4,但是在7天抑制tlr1,在1,5,7天抑制tlr6,
: 在7天抑制tlr8. reviewer问如何解释在不同天数的表达的差异?如何解释这里没有
: kinetics pattern as when these changes will occur?这些数据就是这样的结果,
: 也许当时调整一下数据会得到更好的结果方便解释,可是我保留了较为原始的数据。不
: 知该如何解释?

avatar
f*s
5


【在 f**********r 的大作中提到】
: 老鼠里有TLR8?
:
: 制tlr6,

avatar
f*s
6
牵强么?我一个reviewer就是揪着这点 别的都没啥实质性意见
我在想能不能通过解释不同细菌组分可能释放的时间不一样,比如lps肯定先释放并且
接触细胞,这样tlr4可以再第一天开始就被化合物抑制;最后可能才是细菌single
stand RNA,这样能解释为什么识别ssRNA的tlr8为什么会在第7天被化合物抑制 这样激
活受体的时间也不一样

say
for

【在 a******g 的大作中提到】
: If you don't need experiments to support your explanation, you can just say
: the compound might be metabolized in liver during the period of
: experimentation. The different metabolites of your compound may account for
: the differential activity towards TLRs.
:
: 制tlr6,

avatar
a*g
7
If you can find references to support your claim, it is fine. If there is no
reference to support it, you'd better not argue this way.
Which mouse model are you using? BDL?

【在 f******s 的大作中提到】
: 牵强么?我一个reviewer就是揪着这点 别的都没啥实质性意见
: 我在想能不能通过解释不同细菌组分可能释放的时间不一样,比如lps肯定先释放并且
: 接触细胞,这样tlr4可以再第一天开始就被化合物抑制;最后可能才是细菌single
: stand RNA,这样能解释为什么识别ssRNA的tlr8为什么会在第7天被化合物抑制 这样激
: 活受体的时间也不一样
:
: say
: for

avatar
f*s
8
呵呵 不是肝纤维化的 感染的模型

no

【在 a******g 的大作中提到】
: If you can find references to support your claim, it is fine. If there is no
: reference to support it, you'd better not argue this way.
: Which mouse model are you using? BDL?

avatar
a*g
9
I think arguing about the metabolism of your compound is pretty reasonable,
especially when you don't know the PK and PD of your drug.

【在 f******s 的大作中提到】
: 呵呵 不是肝纤维化的 感染的模型
:
: no

avatar
f*s
10
恩 谢谢回复 我回头和老板讨论讨论吧

,

【在 a******g 的大作中提到】
: I think arguing about the metabolism of your compound is pretty reasonable,
: especially when you don't know the PK and PD of your drug.

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