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a 土博# Biology - 生物学

c*e2012-05-20 07:05

1 楼

两位高手中的高手，
Could you please give answers to my questions from Q39 to Q51?
I do not have answers on these problems. I rely you guys for answers.
Thanks a ton,
CareerChange

e*92012-05-20 07:05

2 楼

朋友家请人杀的 275刀6次
我今天去买sluggo plus 时候顺便买了一瓶
Concentrate Weed B Gon MAX Plus Crabgrass Control
链接是这个，说是浓缩兑水用
http://www.homedepot.com/p/Ortho-32-oz-Concentrate-Weed-B-Gon-M
不知道效果如何。这样还能省点钱。。。。

f*u2012-05-20 07:05

3 楼

会是什么原因？我发现似乎跟我移动笔记本有关，有没有可能是我拿的时候主板变形导
致cpu散热不良？

n*k2012-05-20 07:05

4 楼

I just want to point it out that it doesn't really matter that much when u
are really good, 土博 or not...
Check this guy out:
http://neurobiology.informatics.sunysb.edu/graduate/faculty/sha
What I heard was 3-4 ys into his postdoc training, he never got around to
be on job market since the jobs were looking after him. He had great offer
from several places but chose SUNY stony brook with something like 2M or more package(not
exactly sure though)...
For those looking for a postdoc lab, I think this guy is not a bad choice...
He is from Hongjun Song's lab...he could well be a next HJ Song: very junior
but very sharp and critical so people in the field take notes and respect
that...

M*52012-05-20 07:05

5 楼

很多题其实并不是很难，也不tricky
几个答案一对比就知道它要考的是什么知识点

m*i2012-05-20 07:05

6 楼

最好的办法是手动挖。

p*m2012-05-20 07:05

7 楼

one friend will start his lab in Stanford soon, a tubo from ION

x*y2012-05-20 07:05

8 楼

Hi, I'm not high hand, but I think the right answer is given. You can
discuss about it if you have any doubt...

【在 c**********e 的大作中提到】

: 两位高手中的高手，
: Could you please give answers to my questions from Q39 to Q51?
: I do not have answers on these problems. I rely you guys for answers.
: Thanks a ton,
: CareerChange

T*m2012-05-20 07:05

9 楼

有些效果，但一般不能完全杀死蒲公英。蒲公英额根还会再长出来的。对付蒲公英最重
要的是，不能让种子落在草地里。

【在 e******9 的大作中提到】

: 朋友家请人杀的 275刀6次
: 我今天去买sluggo plus 时候顺便买了一瓶
: Concentrate Weed B Gon MAX Plus Crabgrass Control
: 链接是这个，说是浓缩兑水用
: http://www.homedepot.com/p/Ortho-32-oz-Concentrate-Weed-B-Gon-M
: 不知道效果如何。这样还能省点钱。。。。

g*r2012-05-20 07:05

10 楼

这年头还有人介绍人去跳neuroscience的坑啊？嘿嘿
说实话，最好你们都去做neuroscience

to
offer
not
..
junior

【在 n********k 的大作中提到】

: I just want to point it out that it doesn't really matter that much when u
: are really good, 土博 or not...
: Check this guy out:
: http://neurobiology.informatics.sunysb.edu/graduate/faculty/sha
: What I heard was 3-4 ys into his postdoc training, he never got around to
: be on job market since the jobs were looking after him. He had great offer
: from several places but chose SUNY stony brook with something like 2M or more package(not
: exactly sure though)...
: For those looking for a postdoc lab, I think this guy is not a bad choice...
: He is from Hongjun Song's lab...he could well be a next HJ Song: very junior

d*e2012-05-20 07:05

11 楼

我也觉得你是高手，因为你的解释都是有根有据的

【在 x***y 的大作中提到】

: Hi, I'm not high hand, but I think the right answer is given. You can
: discuss about it if you have any doubt...

e*92012-05-20 07:05

12 楼

密密麻麻的看着都起鸡皮疙瘩。。。

【在 m**i 的大作中提到】

: 最好的办法是手动挖。

f*72012-05-20 07:05

13 楼

sunysb or nyu?

to
offer
not

【在 n********k 的大作中提到】

M*52012-05-20 07:05

14 楼

I agree with you.
Right answers are given for a lot of problems.

【在 x***y 的大作中提到】

: Hi, I'm not high hand, but I think the right answer is given. You can
: discuss about it if you have any doubt...

b*y2012-05-20 07:05

15 楼

如果你有大片的蒲公英想快速让它们消失，用Weed-B-gone是最简单的办法了。接上水
龙头，一定要把蒲公英的叶子完全喷湿。过两三天它们就全枯死了。

n*k2012-05-20 07:05

16 楼

I just refer to the PI and as a 土博...As for the research direction, it is
just like you:))) prefer uglier woman right...BTW, what would you do besides
neuroscience, evolution, aging etc if you are really curious about
bioscience...

【在 g*********r 的大作中提到】

: 这年头还有人介绍人去跳neuroscience的坑啊？嘿嘿
: 说实话，最好你们都去做neuroscience
:
: to
: offer
: not
: ..
: junior

e*92012-05-20 07:05

17 楼

那就好！！哈哈哈希望能成功省钱了！

【在 b**********y 的大作中提到】

: 如果你有大片的蒲公英想快速让它们消失，用Weed-B-gone是最简单的办法了。接上水
: 龙头，一定要把蒲公英的叶子完全喷湿。过两三天它们就全枯死了。

n*k2012-05-20 07:05

18 楼

NY state at stony brook, my bad, thanks...

【在 f*******7 的大作中提到】

: sunysb or nyu?
:
: to
: offer
: not

d*02012-05-20 07:05

19 楼

其实也可以很漂亮。

g*o2012-05-20 07:05

20 楼

neuroscience咋了
听起来多牛逼啊
脑科学啊，啧啧

【在 g*********r 的大作中提到】

: 这年头还有人介绍人去跳neuroscience的坑啊？嘿嘿
: 说实话，最好你们都去做neuroscience
:
: to
: offer
: not
: ..
: junior

l*d2012-05-20 07:05

21 楼

草地不算大嘛，我家花了将近600刀。

【在 e******9 的大作中提到】

f*72012-05-20 07:05

22 楼

2m startup is huge.. what's the common number for neuroscience?

【在 n********k 的大作中提到】

: NY state at stony brook, my bad, thanks...

n*k2012-05-20 07:05

23 楼

As I said, I am not sure about the 2M, but it was a very handsome offer for
sure...he also had(or could have) offer from UCSF etc I believe...so...as
for the routine, somewhere between 0.5-1M I believe...Someone from P's
former lab also had very handsome offer from the school I believe...

【在 f*******7 的大作中提到】

: 2m startup is huge.. what's the common number for neuroscience?

j*t2012-05-20 07:05

24 楼

赞neuroscience, evolution, aging。。。

is
besides

【在 n********k 的大作中提到】

: I just refer to the PI and as a 土博...As for the research direction, it is
: just like you:))) prefer uglier woman right...BTW, what would you do besides
: neuroscience, evolution, aging etc if you are really curious about
: bioscience...

z*o2012-05-20 07:05

25 楼

不管大家愿不愿意，土博将越来越多的出现在米国大学的faculty list上。

【在 n********k 的大作中提到】

g*02012-05-20 07:05

26 楼

neuroscience和cancer，两个苦逼的不能再苦逼的方向。
共同特点是搞的人巨多，但搞出来的东西又经不起实践检验，一点前景（钱景）也没有
。

n*k2012-05-20 07:05

27 楼

well, pretty much everything you said is true. In fact, I pointed out
pretty much the same to all student/postdoc candidates I have interviewed/
chatted with so far and ask them why they choose bio and what their life
passions and career goals...again since man(woman:))) too) are colorful,
The GuaGua might prefer the beauty without a brain while guagua1220 could
favor deep thinker over a beauty, agreed?

【在 g********0 的大作中提到】

: neuroscience和cancer，两个苦逼的不能再苦逼的方向。
: 共同特点是搞的人巨多，但搞出来的东西又经不起实践检验，一点前景（钱景）也没有
: 。

p*e2012-05-20 07:05

28 楼

大大可不可以谈谈对brainbow的看法，最近看了几篇相关的文章，感觉想法很好。

【在 n********k 的大作中提到】

: well, pretty much everything you said is true. In fact, I pointed out
: pretty much the same to all student/postdoc candidates I have interviewed/
: chatted with so far and ask them why they choose bio and what their life
: passions and career goals...again since man(woman:))) too) are colorful,
: The GuaGua might prefer the beauty without a brain while guagua1220 could
: favor deep thinker over a beauty, agreed?

n*k2012-05-20 07:05

29 楼

I have quite some interest in brainbow tech and theoretically it could have
some great usages...however, surprisingly I haven't seen it got picked up
widely(yet?), not sure about the reasons....many tech sounds great on paper
but in practice has lots of caveats or challenges...for many working with
Cre-lines, they would know what I mean...gosh, in our field, one has to be
very cautious and cannot just trust a study and choose a cre-line without
careful preliminary experiments and/or knowing beyond the surface level,
otherwise one could waste ys of work...And this is not uncommon and some
actually publish their studies without even (intentionally or
unintentionally) knowing the caveats...
Not sure whether this would be the case with brainbow...for similar reasons
(on CLIP and nuclear run on assay), I had cautions on CLIP/RIP-seq,
GRO-Seq but seems to be unnecessary...I would too very much
appreciate comments from any who has hands on experience on this tech...hey, P---have you heard of anything on this front?

【在 p*****e 的大作中提到】

: 大大可不可以谈谈对brainbow的看法，最近看了几篇相关的文章，感觉想法很好。

p*e2012-05-20 07:05

30 楼

确实，brainbow2007年就出来了，到现在好像就几个lab在做。我感觉如果把这个
方法引入到其他器官中，会是一个非常有用的工具。
我没有接触过cre，不知道为什么cre-line需要特别小心，是不是off-target的原
因？
关于CLIP-Seq，我在miRNA里面看到好多人在用，感觉结论还算互相能论证，但是
在lncRNA里面，问题比较多，怀疑二级结构太复杂。
小白，胡乱说的。

have
paper

【在 n********k 的大作中提到】

: I have quite some interest in brainbow tech and theoretically it could have
: some great usages...however, surprisingly I haven't seen it got picked up
: widely(yet?), not sure about the reasons....many tech sounds great on paper
: but in practice has lots of caveats or challenges...for many working with
: Cre-lines, they would know what I mean...gosh, in our field, one has to be
: very cautious and cannot just trust a study and choose a cre-line without
: careful preliminary experiments and/or knowing beyond the surface level,
: otherwise one could waste ys of work...And this is not uncommon and some
: actually publish their studies without even (intentionally or
: unintentionally) knowing the caveats...

n*k2012-05-20 07:05

31 楼

For Cre-line: the leakiness, the efficiency, the toxicity, the variability
etc etc could all easily defeat one's experimental design...
For CLIP, it took so many ys for the field to pick up the tech since Robert
Darnell's group first reported in science...still not nearly as widely as I
would predict but maybe it is because I have a strong interest in RNA
biology...

【在 p*****e 的大作中提到】

: 确实，brainbow2007年就出来了，到现在好像就几个lab在做。我感觉如果把这个
: 方法引入到其他器官中，会是一个非常有用的工具。
: 我没有接触过cre，不知道为什么cre-line需要特别小心，是不是off-target的原
: 因？
: 关于CLIP-Seq，我在miRNA里面看到好多人在用，感觉结论还算互相能论证，但是
: 在lncRNA里面，问题比较多，怀疑二级结构太复杂。
: 小白，胡乱说的。
:
: have
: paper

b*s2012-05-20 07:05

32 楼

原来是科大的师兄。再一看实验室member，发现原来的同学，他实验室的师弟成了他的
薄厚。
他这么牛？2M?

to
offer
more package(not
..
junior

【在 n********k 的大作中提到】

s*r2012-05-20 07:05

33 楼

不管是CLIP还是Nuclear Run on，加上sequencing，
都还是一种Screen吧。

For Cre-line: the leakiness, the efficiency, the toxicity, the variability
etc etc could all easily defeat one's experimental design...
For CLIP, it took so many ys for the field to pick up the tech since Robert
Darnell's group first reported in science...still not nearly as widely as I
would predict but maybe it is because I have a strong interest in RNA
biology...

【在 n********k 的大作中提到】

: For Cre-line: the leakiness, the efficiency, the toxicity, the variability
: etc etc could all easily defeat one's experimental design...
: For CLIP, it took so many ys for the field to pick up the tech since Robert
: Darnell's group first reported in science...still not nearly as widely as I
: would predict but maybe it is because I have a strong interest in RNA
: biology...

n*k2012-05-20 07:05

34 楼

Yes, he is from UTSC--BA-PHD, physics major...if you have a chance, you
shall talk to him...I am not saying he is exceptional but he is as good if
not better as any of the top folks from our board...He is building up his
records and GIVEN CHANCES, he could well be a next HJ Song...

【在 b******s 的大作中提到】

: 原来是科大的师兄。再一看实验室member，发现原来的同学，他实验室的师弟成了他的
: 薄厚。
: 他这么牛？2M?
:
: to
: offer
: more package(not
: ..
: junior

j*w2012-05-20 07:05

35 楼

土博怎么了？有些土博是水平差，或者开窍晚，当年没法出国。有些土博是由于种种原
因没出国，本身很强。完全不能放在一起比。你强，总会出头。你差，也别怨天尤人了
，没用的。

r*j2012-05-20 07:05

36 楼

本科并不是在科大念的。

【在 n********k 的大作中提到】

: Yes, he is from UTSC--BA-PHD, physics major...if you have a chance, you
: shall talk to him...I am not saying he is exceptional but he is as good if
: not better as any of the top folks from our board...He is building up his
: records and GIVEN CHANCES, he could well be a next HJ Song...

p*m2012-05-20 07:05

37 楼

lz本来就是再夸土搏好吧。。

【在 j***w 的大作中提到】

: 土博怎么了？有些土博是水平差，或者开窍晚，当年没法出国。有些土博是由于种种原
: 因没出国，本身很强。完全不能放在一起比。你强，总会出头。你差，也别怨天尤人了
: ，没用的。

j*w2012-05-20 07:05

38 楼

对。我完全同意lz。

t*82012-05-20 07:05

39 楼

a little correction: his BA is not from USTC, and his PhD is biophysics, his
startup is less than 1M, and he was on job market for 1-2 yrs.
:)

【在 n********k 的大作中提到】

n*k2012-05-20 07:05

40 楼

Thanks...seems I got everything wrong...what I heard was the department
bought him some major instrument including confocal aside from his start-up.
..As for the job market, what I have heard is rather different and it is not
quite proper to elaborate here...Anyway, let me rephrase a bit: for him,
job or not wasn't a concern...

his

【在 t******8 的大作中提到】

: a little correction: his BA is not from USTC, and his PhD is biophysics, his
: startup is less than 1M, and he was on job market for 1-2 yrs.
: :)