Does anybody know whether pmaxGFP can be used in flow cytometry analysis? - 未名空间MITBBS历史存档

国际科技财经博客移民网络热点娱乐民生时事公众号

Redian新闻

>未名空间

>Biology - 生物学

Does anybody know whether pmaxGFP can be used in flow cytometry analysis?

Does anybody know whether pmaxGFP can be used in flow cytometry analysis?# Biology - 生物学

A*r2012-07-13 07:07

1 楼

生物PhD学生办绿卡的经历 by yeye
http://www.mitbbs.com/article/Immigration/14500119_3.html
http://www.mitbbs.com/article/Immigration/14500173_3.html
http://www.mitbbs.com/article/Immigration/14500415_3.html
http://www.mitbbs.com/article/Immigration/14509753_3.html
http://www.mitbbs.com/article/Immigration/14509798_3.html

m*e2012-07-13 07:07

2 楼

我们还要盖很多……

q*02012-07-13 07:07

3 楼

Recently, I transfected pmaxGFP plasmid to HepG2 cells by using
Lipofectamine2000 to track the positive-transfected cells with another test
plasmid. However, the FACS result showed no separation between GFP positive
or negative cells, only one cell population. I don't know whether the
transfection efficiency is too high that almost every cell was transfected?
or pmaxGFP is not a proper GFP plasmid to use in FACS? Your suggestions will
be appreciated.

d*a2012-07-13 07:07

4 楼

嗯，就看出不出ipad mini高清版了。

a*d2012-07-13 07:07

5 楼

it should work for flow
make sure you have no-transfected control population
do several different transfection dose may also help to tell the difference

m*a2012-07-13 07:07

6 楼

据说从套子测的size变厚了，是因为换了retina

【在 d***a 的大作中提到】

: 嗯，就看出不出ipad mini高清版了。

q*02012-07-13 07:07

7 楼

Thanks.

difference

【在 a****d 的大作中提到】

: it should work for flow
: make sure you have no-transfected control population
: do several different transfection dose may also help to tell the difference