大家来谈谈这篇最新的CRISPR文章# Biology - 生物学
h*y
1 楼
Jaenisch lab最新发表的利用CRISPR同时敲除5个基因的文章。CRISPR前途无限啊。再搞
出几个inducible的CRISPR就更厉害了。
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/C
as-Mediated Genome Engineering
http://www.ncbi.nlm.nih.gov/pubmed/23643243
Cell. 2013 May 1. pii: S0092-8674(13)00467-4. doi: 10.1016/j.cell.2013.04.02
5.
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/C
as-Mediated Genome Engineering.
Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R.
Source
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Abstract
Mice carrying mutations in multiple genes are traditionally generated by seq
uential recombination in embryonic stem cells and/or time-consuming intercro
ssing of mice with a single mutation. The CRISPR/Cas system has been adapted
as an efficient gene-targeting technology with the potential for multiplexe
d genome editing. We demonstrate that CRISPR/Cas-mediated gene editing allow
s the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty - 8 allele
s) in mouse embryonic stem (ES) cells with high efficiency. Coinjection of C
as9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes
generated mice with biallelic mutations in both genes with an efficiency of
80%. Finally, we show that coinjection of Cas9 mRNA/sgRNAs with mutant olig
os generated precise point mutations simultaneously in two target genes. Thu
s, the CRISPR/Cas system allows the one-step generation of animals carrying
mutations in multiple genes, an approach that will greatly accelerate the in
vivo study of functionally redundant genes and of epistatic gene interactio
ns.
出几个inducible的CRISPR就更厉害了。
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/C
as-Mediated Genome Engineering
http://www.ncbi.nlm.nih.gov/pubmed/23643243
Cell. 2013 May 1. pii: S0092-8674(13)00467-4. doi: 10.1016/j.cell.2013.04.02
5.
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/C
as-Mediated Genome Engineering.
Wang H, Yang H, Shivalila CS, Dawlaty MM, Cheng AW, Zhang F, Jaenisch R.
Source
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.
Abstract
Mice carrying mutations in multiple genes are traditionally generated by seq
uential recombination in embryonic stem cells and/or time-consuming intercro
ssing of mice with a single mutation. The CRISPR/Cas system has been adapted
as an efficient gene-targeting technology with the potential for multiplexe
d genome editing. We demonstrate that CRISPR/Cas-mediated gene editing allow
s the simultaneous disruption of five genes (Tet1, 2, 3, Sry, Uty - 8 allele
s) in mouse embryonic stem (ES) cells with high efficiency. Coinjection of C
as9 mRNA and single-guide RNAs (sgRNAs) targeting Tet1 and Tet2 into zygotes
generated mice with biallelic mutations in both genes with an efficiency of
80%. Finally, we show that coinjection of Cas9 mRNA/sgRNAs with mutant olig
os generated precise point mutations simultaneously in two target genes. Thu
s, the CRISPR/Cas system allows the one-step generation of animals carrying
mutations in multiple genes, an approach that will greatly accelerate the in
vivo study of functionally redundant genes and of epistatic gene interactio
ns.
