Redian新闻
>
questions about mMESSENGERmMACHINE sp6
avatar
questions about mMESSENGERmMACHINE sp6# Biology - 生物学
j*3
1
biotech方向 哪个学院更好?除了soft/hard money的区别以外 其他有没有特别的不同
?medical school是不是funding压力会很大?
avatar
w*r
2
童鞋们,
首先祝愿大家绿卡申请顺利。
我打算自己提交EB-1B,petition letter 是以单位领导的口气写的。现
在想 efile and PP. 有些问题不是很清楚,请大家有经验的或者知道如何解决的帮助
一下。
1,在选择是自己为自己申请还是选择attorney 申请呢?
2,请问140还需不需要单位签字
3,请问其中的A#应该是在哪里找到啊?
多谢你们!
avatar
h*g
3
I am using Ambion mMESSENGERmMACHINE Sp6 to make mRNA from plasmid. After
making mRNA, I run a simple agarose gel. There are two bands. One band is
not very close to the actual size (400bp smaller), there is also another
smaller and stronger band. Can I still use the mRNA? Why there are two bands
? Thanks.
avatar
c*7
4
看你能从naf还是nih拿grant 了

【在 j******3 的大作中提到】
: biotech方向 哪个学院更好?除了soft/hard money的区别以外 其他有没有特别的不同
: ?medical school是不是funding压力会很大?

avatar
s*h
5
EB-1B 能DIY吗?我的影响是必须找lawyer.
avatar
x*w
6
Did you purify mRNA before electrophoresis?
Un-incorporated NTP also could be detected by EB.
avatar
w*y
7
不知道答案,但是帮你顶一下。如果成功了请一定告诉大家一下经验!
140应该不需要单位签字了,因为你是efile,suppose 应该是单位来file, 是internet
agree.
avatar
h*g
8
I did not purify mRNA. Could you tell me how to purify it? Using what kit?
Thanks!

【在 x**w 的大作中提到】
: Did you purify mRNA before electrophoresis?
: Un-incorporated NTP also could be detected by EB.

avatar
r*n
9
Did you use denature gel? RNA gel? Normal DNA gels may not give you good
estimation on the size. Purification I used RNAeasy kit from Qiagen, works
well. You could use classic pheno-chloroform extraction if you don't have
the kit. If your RNA coded product has fluorescence or very specific
phenotypes in your system maybe a direct functional verification is easier.
good luck
avatar
c*r
10
mMESSENGER mMACHINE 说明书里提供了好像是三种纯化方法。试剂盒里也有一些纯化所
需的试剂。

【在 h*********g 的大作中提到】
: I did not purify mRNA. Could you tell me how to purify it? Using what kit?
: Thanks!

avatar
x*w
11
My experience is LiCl +isopropanol works perfect.
avatar
h*g
12
Thanks. I used normal DNA gel. I don't know why there two bands. Thanks.

【在 r********n 的大作中提到】
: Did you use denature gel? RNA gel? Normal DNA gels may not give you good
: estimation on the size. Purification I used RNAeasy kit from Qiagen, works
: well. You could use classic pheno-chloroform extraction if you don't have
: the kit. If your RNA coded product has fluorescence or very specific
: phenotypes in your system maybe a direct functional verification is easier.
: good luck

avatar
s*n
13
Did you digest the DNA template? Did you run a formaldehyde gel? Did you use
RNA ladder?
RNA runs at lower MW comparing to dsDNA; if you are not using RNA gel, your
RNA will be easily degraded.
相关阅读
logo
联系我们隐私协议©2024 redian.news
Redian新闻
Redian.news刊载任何文章,不代表同意其说法或描述,仅为提供更多信息,也不构成任何建议。文章信息的合法性及真实性由其作者负责,与Redian.news及其运营公司无关。欢迎投稿,如发现稿件侵权,或作者不愿在本网发表文章,请版权拥有者通知本网处理。