Redian新闻
>
Re: why this E.Coli grow so slow?
avatar
Re: why this E.Coli grow so slow?# Biology - 生物学
c*t
1
Perfectly normal.
The bacterial was not in growth phase. It could be caused by
1. high cell density. O.D. can mislead you sometimes because
that can vary for different strains. But don't people
usually use OD 600 (598) to check cell density?
2. the cells were in cold for a period of time.
3. lack of oxygen.
avatar
R*s
2
1. never heard of measuring bacterial at OD546 and calculating as u
said below. We only do it at OD600.
2. What temperature you grow the cells? Hope it is 37, not 30.
BL21(DE3)Plyss does grow relatively slower than other E.Coli strains.
However, it is not as slow as yours.
3. In fact, 12 hours for your transformed culture to reach OD 0.8 is not
terribly slow. It usually take us 6-9 hours to do so.
4. Last suggestion, make sure your Amp and Chl concentration is correct.
Even if they are correct,
相关阅读
logo
联系我们隐私协议©2024 redian.news
Redian新闻
Redian.news刊载任何文章,不代表同意其说法或描述,仅为提供更多信息,也不构成任何建议。文章信息的合法性及真实性由其作者负责,与Redian.news及其运营公司无关。欢迎投稿,如发现稿件侵权,或作者不愿在本网发表文章,请版权拥有者通知本网处理。