Re: 怎样调整gel，走好SDS-PAGE?# Biology - 生物学
M*e
1 楼
throw in a couple of points:
when you run an SDS-PAGE, you try to fractinate the protein
by the matrix of acrylamide. so the qualities of both the
gel and the samples are important to determine migration.
basically, do not use gels that have been stored for a long
time, use fresh AP and high-quality of acrylamide to make a
PAGE gel, giving long time for the bottom separation gel to
polymerize; or, just buy a commercial gel from BioRad, either
non-gradient or gradient (this one seems to be better
when you run an SDS-PAGE, you try to fractinate the protein
by the matrix of acrylamide. so the qualities of both the
gel and the samples are important to determine migration.
basically, do not use gels that have been stored for a long
time, use fresh AP and high-quality of acrylamide to make a
PAGE gel, giving long time for the bottom separation gel to
polymerize; or, just buy a commercial gel from BioRad, either
non-gradient or gradient (this one seems to be better