Re: 请大家帮忙# Biology - 生物学
M*e
1 楼
TUNEL labeling of tissue slides would tell you roughly how many apoptotic
cells are present on the slides. or you can try Hoechst staining for DNA
fragmentation. apoptotic cells are stained more bright for Hoechst, which is
thought due to change of chromatin structure. this is usually done with
propidium iodide staining, which stains dead cells bright. so apoptotic cell
is propidium iodide dim, and Hoechst bright. membrane structure change occurs
during apoptosis, and annexin V binds specificall
cells are present on the slides. or you can try Hoechst staining for DNA
fragmentation. apoptotic cells are stained more bright for Hoechst, which is
thought due to change of chromatin structure. this is usually done with
propidium iodide staining, which stains dead cells bright. so apoptotic cell
is propidium iodide dim, and Hoechst bright. membrane structure change occurs
during apoptosis, and annexin V binds specificall