Re: 跨膜蛋白的orientation? - 未名空间MITBBS历史存档

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Re: 跨膜蛋白的orientation?

Re: 跨膜蛋白的orientation?# Biology - 生物学

s*e2002-12-24 08:12

1 楼

(1)
fusiong protein with a enzyme digestion site. overexpress it and digest.
check media by western.
(2)
EGFP fused to N tel. microscopy
(3)
homology search of the N-ter from database.
(4)
I don't know. :)

s*y2002-12-24 08:12

2 楼

Search the amino acid sequence on data base for signal peptide.
MOst signal peptides have been characterized.
I think, roughly, but not in detail: digest the whole cell or the membrane
vesicles make from supersonic, without using any detergent. If a pepetide is
outside membrane, it will be digested. If it's inside, it will not.
GFlycosylation should use similar mechanism, but instead of protease,
glycosylase is used to labled the outside peptide, then a mess spec can be
used to determined the l