Re: 想问做电生理的大牛一个问题# Biology - 生物学
r*e
1 楼
Seal test is valid under one condition: not much ionic currents at your
holding potential. So if you recording excitable cells ( muscle or neuron),
you need to use holding potential -60mV. If you use 0mV, you will get
something like 60 Mohm. Similarly, if you have channels expressed (active) at
0 mV, your seal test at 0 mV holding potential will look bad. In both cases,
there may not be any leakage.
holding potential. So if you recording excitable cells ( muscle or neuron),
you need to use holding potential -60mV. If you use 0mV, you will get
something like 60 Mohm. Similarly, if you have channels expressed (active) at
0 mV, your seal test at 0 mV holding potential will look bad. In both cases,
there may not be any leakage.